驴源罗伊氏乳杆菌的安全性评价  

Safety Evaluation of Lactobacillus reuteri Isolated from Donkey’s Intestine

作　　者：王卓[1] 冯培祥 李光玉[1] 刘晓颖[1] 郭肖兰 司华哲 姜桂苗[2,3] 赵付伟 刘晗璐 WANG Zhuo;FENG Pei-xiang;LI Guang-yu;LIU Xiao-ying;GUO Xiao-lan;SI Hua-zhe;JIANG Gui-miao;ZHAO Fu-wei;LIU Han-lu(State Key Laboratory of Special Economic Animal Molecular Biology,Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences,Changchun 130112,China;Collaborative Innovation Center of Industrial Technology,Liaocheng University,Liaocheng 252000,China;Engineering Research Center of Gelatin-based Traditional Chinese Medicine,Dong-E-E-Jiao Co.,Ltd.,Liaocheng 252201,China)

机构地区：[1]中国农业科学院特产研究所,吉林省特种经济动物分子生物学省部共建国家重点实验室,吉林长春130112 [2]聊城大学驴产业科技协同创新中心,山东聊城252000 [3]国家胶类中药工程技术研究中心,东阿阿胶股份有限公司,山东聊城252201

出　　处：《特产研究》2020年第3期39-43,共5页Special Wild Economic Animal and Plant Research

基　　金：中国农业科学院科技创新工程专项经费(CAAS-ASTIP-2020-ISAPS);吉林省科技发展计划项目(20200301019RQ)。

摘　　要：对驴肠道内分离鉴定的罗伊氏乳杆菌L2-2株安全性进行评价,以确定其能否作为益生菌菌株并用于后续的产品研发。通过小鼠大剂量L2-2株经口毒性试验,记录小鼠增重和采食量,检测脏器指数、血液学指标、细菌易位情况。所有试验小鼠无异常表现,体重、采食量均正常,灌胃罗伊氏乳杆菌可增加小鼠胸腺指数和血清球蛋白含量,降低血脂含量,无细菌易位现象。本研究初步证明罗伊氏乳杆菌L2-2对小鼠安全、无毒副作用,且能增强免疫功能。This work evaluated the safety of Lactobacillus reuteri L2-2 isolated from donkey’s intestine to determine whether it could be used as probiotic strains for the subsequent product development.Through oral toxicity test of large dose of L2-2 strain to mice,the weight gain and feed intake of mice were recorded,and organ index,hematological indices and bacterial translocation situations were detected.All test mice had no abnormal performance,the body weight and feed intake were normal.Intragastric administration of L.reuteri could increase the thymus index and globulin content in serum,reduce triglyceride content.No bacterial translocation was detected.It was preliminarily proved that L.reuteri L2-2 was safe,no-toxic to mice and it could also enhance the immunity functions.

关 键 词：驴源 罗伊氏乳杆菌 安全性 

分 类 号：S816.3[农业科学—饲料科学]