应用酶标仪测定饮用水中碘含量的方法研究  

Study on the application of enzyme-labeled instrument in the detection of iodine in drinking water

作　　者：徐署东 李卫东[1] 赵立胜[1] 刘婷婷[1] 姜静静[1] 许娴[1] 虞晨[1] 田翠翠[1] 张滔[1] Xu Shudong;Li Weidong;Zhao Lisheng;Liu Tingting;Jiang Jingjing;Xu Xian;Yu Chen;Tian Cuicui;Zhang Tao(Department for Endemic and Parasitic Diseases Control and Prevention,Anhui Center for Disease Control and Prevention,Hefei 230601,China)

机构地区：[1]安徽省疾病预防控制中心地方病与寄生虫病防治科,合肥230601

出　　处：《中华地方病学杂志》2020年第1期64-68,共5页Chinese Journal of Endemiology

摘　　要：目的建立饮用水中碘含量的酶标仪自动比色检测方法(以下简称本方法)。方法在0~10μg/L和0~100μg/L两个碘质量浓度范围内测定水碘含量,对方法的线性关系及检出限、精密度、准确度进行实验验证。并与国家碘缺乏病参照实验室推荐的砷铈催化分光光度法水碘测定结果进行比较分析。结果0~10μg/L和0~100μg/L范围内,│r│均>0.9990,检出限分别为0.6、1.1μg/L(取样量分别为200、100μl);低、中、高3种碘质量浓度水样检测精密度相对标准偏差(RSD)均<3%,回收率范围分别为92.5%~108.3%和93.2%~108.9%,总平均回收率分别为100.0%和100.3%。分别应用本方法和砷铈催化分光光度法在0~10μg/L和0~100μg/L范围内检测40份水样,水碘含量比较差异无统计学意义(t=0.99,P>0.05)。结论本方法应用于水碘含量测定具有较好的标准曲线线性关系,精密度好,准确度高,适合推广应用。Objective To establish an automatic colorimetric method for determination of iodine in drinking water by enzyme-labeled instrument(hereinafter referred to as this method).Methods The water iodine was measured in the range of 0-10μg/L and 0-100μg/L,experiments were carried out on linear relationship,detection limit,precision and accuracy of this method.And the results were compared with the National Reference Laboratory for Iodine Deficiency Disorders recommended arsenic cerium catalytic spectrophotometry method.Results In the range of 0-10μg/L and 0-100μg/L,all│r│>0.9990,the detection limits were 0.6 and 1.1μg/L(samples were 200 and 100μl),respectively;the relative standard deviation(RSD)of water samples of low,medium and high iodine mass concentrations were<3%,the recovery rates ranged from 92.5%to 108.3%and 93.2%to 108.9%,with a total average recovery of 100.0%and 100.3%,respectively.This method and arsenic cerium catalytic spectrophotometry method were used to detect 40 water samples in the range of 0-10μg/L and 0-100μg/L,there was no significant difference in water iodine content between the two methods(t=0.99,P>0.05).Conclusion This method has good linear curve relationship for determination of water iodine content,good precision and high accuracy,and it is suitable for wide application.

关 键 词：水 碘 酶标仪 测定范围 检测限 

分 类 号：R1[医药卫生—公共卫生与预防医学]