Differentially expressed miRNAs in anthers may contribute to the fertility of a novel Brassica napus genic male sterile line CN12A  

作　　者：DONG Yun WANG Yi JIN Feng-wei XING Lijuan FANG Yan ZHANG Zheng-ying ZOU Jun-jie WANG Lei XU Miao-yun 

机构地区：[1]Crop Research Institute,Gansu Academy of Agricultural Sciences,Lanzhou 730070,P.R.China [2]National Key Facility for Crop Gene Resources and Genetic ImprovementBiotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China [3]Gansu Provincial Key Lab of Aridland Crop Sciences/Gansu Agricultural University,Lanzhou 730070.P.R.China

出　　处：《Journal of Integrative Agriculture》2020年第7期1731-1742,共12页农业科学学报（英文版）

基　　金：This project was supported by the National Key Research Program of China(2018YFD0100500);the Major Projects for New Varieties of Genetically Modified Organisms,China(2018ZX0801109B);the Agricultural Science and Technology Innovation Project of Gansu Academy of Agricultural Sciences,China(2018GAAS04).

摘　　要：In Brassica napus L.(rapeseed),complete genic male sterility(GMS)plays an important role in the utilization of heterosis.Although microRNAs(miRNAs)play essential regulatory roles during bud development,knowledge of how GMS is regulated by miRNAs in rapeseed is rather limited.In this study,we obtained a novel recessive GMS system,CN12AB.The sterile line CN12A has defects in tapetal diferentiation and degradation.llumina sequencing was employed to examine the expression of miRNAs in the buds of CN12A and the fertile line CN12B.We identifed 85 known miRNAs and 120 novel miRNAs that were expressed during rapeseed anther development.When comparing the expression levels of miRNAs between CN12A and CN12B,19 and 18 known miRNAs were found to be difrentially expressed in 0.5--1.0 mm buds and in 2.5--3.0 mm buds,respectively.Among these,the expression levels of 14 miRNAs were higher and the levels of 23 miRNAs were lower in CN12A compared with CN12B.The predicted target genes of these iferentially expressed miRNAS encode protein kinases,F-box domain-containing proteins,MADS-box family proteins,SBP-box gene family members,HD ZIP proteins,floral homeotic protein APETAL A 2(AP2)。and nuclear factor Y,subunit A.These targets have previously been reported to be involved in pollen development and male sterility,suggesting that miRNAs might act as regulators of GMS in rapeseed anthers.Furthermore,RT-qPCR data suggest that one of the dffrentially expressed miRNAs,bna-miR159,plays a role in tapetal differentiation by regulating the expression of transcription factor BnMYB101 and participates in tapetal degradation and infuences callose degradation by manipulating the expression of BnA6.These findings contibute to our understanding of the roles of miRNAs during anther development and the occurrence of GMS in rapeseed.

关 键 词：Brassica napus L. genic male sterility MIRNAS targets molecular regulation network 

分 类 号：S565.4[农业科学—作物学]