Effect of three insect-resistant maizes expressing Cry1le,Cry1Ab/Cry2Aj and Cry1Ab on the growth and development of armyworm Mythimna separata(Walker)  

作　　者：SU Hong-hua JIANG Tao SUN Yu GU Hui-jie WU Jiao-jiao YANG Yi-zhong 

机构地区：[1]School of Horticulture and Plant Protection,Yangzhou University,Yangzhou 225009,P.R.China

出　　处：《Journal of Integrative Agriculture》2020年第7期1842-1849,共8页农业科学学报（英文版）

基　　金：We thank Dr.Jeannie McDonald(Ecology and Evolutionary Biology Department,Cornell University,NY,USA)very much for herlcontribution to the manuscript revision.This research was supported by the National Science and Technology Major Project,China(2016ZX08012-004-010);the Key Research and Development Project of Jiangsu Province,China(BE2017379-3).

摘　　要：Three transgenic maize events(IE09S034,Shuangkang 12--5 and C0030.3.5)produced Cry1le,Cry1Ab/Cry2Aj and G10-EPSPS,Cry1Ab and EPSPS,respectively,all of which target the Asian corn borer.The oriental armyworm Mythimna separata(Walker)is the secondary target.In this study,the effects of the three Bt maizes on the development and survival of armyworm were studied.The results showed that IE09S034 had insecticidal activity against 1st instar larvae,and the survival rate of armyworm fed with Bt maize for 10 days was 462%,significantly lower than that of the control.The larvae at 3rd--6th instar were more tolerant of the Bt toxin than the early instar larvae.However,Shuangkang 12-5 had good insecticidal activity against 1st-5th instar larvae.The mortality was nearly 100%when the larvae were fed with Shuangkang 12-5 before 3rd instar,and the toxin had quick acting eficacy.This event significantly ihibited the development of armyworm;that is,the larval duration of the 3rd and 4th instar larvae fed with Shuangkang 12-5 was prolonged by 4.5 and 3.0 days,respectively.The pupal weight and egg number were also significantly lower than those of the control.For C0030.3.5,it could control 1st--5th instar larvae effectively.The mortality rates were all over 50%if 1st-3rd larvae were fed with this event.The pupal weight of 4th-6th instar larvae fed with Bt maize were only 53.9,56.8 and 54.6%,respectively,compared to that of the control.The number of eggs laid was significantly less than the control.The results indicate that all three transgenic maize events exhibit the potential to provide effective control of early instar larvae of armyworm.which can be commercialized in future to control lepidoptera pests such as Asian corn borer and armyworm.

关 键 词：transgenic maize ARMYWORM development survival 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]