基于二氧化锆和金纳米粒子的葡萄糖生物传感器  

Glucose biosensor based on zirconium dioxide and gold nanoparticles

作　　者：金丹莉 尹晓蝶 张博 陈小燕 赵文雅 朱冠阳 顾颖颖[1] 安雅睿[1] JIN Danli;YIN Xiaodie;ZHANG Bo;CHEN Xiaoyan;ZHAO Wenya;ZHU Guanyang;GU Yingying;AN Yarui(College of Science,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学理学院,上海200093

出　　处：《实验室科学》2020年第2期56-60,共5页Laboratory Science

基　　金：国家自然科学基金项目(项目编号:21505092);上海理工大学“教学成果奖”培育项目(项目编号:10-18-113-007);上海理工大学教师教学发展研究重点资助项目(项目编号:CFTD18009Z)。

摘　　要：将合成的二氧化锆纳米材料修饰到玻碳电极上面。在此基础上,还修饰了金纳米颗粒,构建了电化学生物传感器用来检测葡萄糖。纳米结构的二氧化锆作为金属氧化物,不仅生物相容性好,而且灵敏度高。此外,修饰的金纳米颗粒可以提高导电性,促进固定化的蛋白质和电极基质之间的电子转移。结果发现葡萄糖的检测范围是0.01~20.0 mmol/L,检测线为7.52μmol/L,并且响应速度快,表明合成的材料对葡萄糖的成功检测。We synthesized zirconium dioxide nanomaterials and modified them into glassy carbon electrodes.On this basis,we also modified gold nanoparticles to construct an electrochemical biosensor to detect glucose.As a metal oxide,nanostructured zirconium dioxide is not only biocompatible,but also highly sensitive.In addition,the modified gold nanoparticles can improve electrical conductivity and promote electron transfer between the immobilized protein and the electrode substrate.We found that the range of glucose detection was 0.01 to 20.0 mmol/L,the detection limit was 7.52μmol/L,and the response time was fast,indicating the successful application of our synthetic materials for the detection of glucose.

关 键 词：二氧化锆 金纳米颗粒 葡萄糖 生物传感器 

分 类 号：O657.1[理学—分析化学]