高血压亚急症患者血管功能异常及与循环内皮祖细胞功能变化的关系  

The relationship between vascular dysfunction and change of functional activities of circulating endothelial progenitor cells in hypertensive urgency

作　　者：彭智华 古钎林 黄振华[2] 詹红[2] 杨震[1,2,3] PENG Zhihua;GU Qianlin;HUANG Zhenhua;ZHAN Hong;YANG Zhen(Department of Cardiology,the First Affiliated Hospital,Sun Yat-Sen University,Guangzhou,Guangdong 510080,China;Department of Emergency,the First Affiliated Hospital,Sun Yat-Sen University,Guangzhou,Guangdong 510080,China;Key Laboratory of Assisted Circulation,Ministry of Health,Guangzhou,Guangdong 510080,China)

机构地区：[1]中山大学附属第一医院心内科,广东省广州市510080 [2]中山大学附属第一医院急诊科,广东省广州市510080 [3]卫生部辅助循环重点实验室,广东省广州市510080

出　　处：《中国动脉硬化杂志》2020年第6期507-512,共6页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金资助项目(81670220);广东省科技计划项目(2015A020212013);广州市科技计划项目(201803010008);中山大学青年教师培育项目(17ykzd18);广州经济技术开发区国际科技合作项目(2017GH13)。

摘　　要：目的探讨高血压亚急症患者循环内皮祖细胞(EPC)功能的改变以及一氧化氮(NO)、肱动脉血流介导的内皮依赖性舒张功能(FMD)与EPC功能的关系。方法招募高血压亚急症患者13例,血压正常者20例(对照组),取外周血提取原代EPC培养后以Transwell小室和CCK-8法评估EPC的迁移、黏附和增殖能力,通过检测FMD、血浆和EPC分泌的NO、粒-巨噬细胞集落刺激因子(GM-CSF)、血管内皮生长因子(VEGF)、白细胞介素6(IL-6)水平评估血管内皮功能。结果高血压亚急症组与对照组相比,EPC细胞功能(包括迁移、增殖和黏附能力)明显下降(P<0.05);血浆及EPC分泌的NO水平有明显差异(P<0.05);血浆及EPC分泌NO水平均与EPC迁移、黏附和增殖能力呈明显的线性关系(P<0.05);FMD与EPC迁移、黏附和增殖能力具有明显相关性(P<0.05)。两组血浆和EPC分泌的GM-CSF、VEGF、IL-6水平均无明显差异(P>0.05)。结论相对血压正常者,高血压亚急症人群的EPC功能(迁移、黏附和增殖)明显下降,且NO水平和FMD均与EPC功能正相关。Aim To investigate the relationship between vascular dysfunction and change of functional activities of circulating endothelial progenitor cells( EPC) in hypertensive urgency. Methods Thirteen hypertensive urgency patients( n = 13) and twenty normotension( n = 20) were recruited for the research. First of all,blood samples were collected and circulating EPC were isolated and cultured. The migration,proliferation and adhesion activities of circulating EPC were detected with Transwell method and CCK-8 assay respectively. In addition,the level of granulocyte-macrophage colony stimulating factor( GM-CSF),vascular endothelial growth factor( VEGF) and interleukin-6( IL-6) from both plasma and EPC production were measured by ELISA. The level of nitric oxide( NO) from both plasma and EPC production were determined by nitrate reductase method. Finally,brachial artery-based flow mediated dilatation( FMD) was used to determine vascular endothelial function. Results Compared with the normotension group,the migration,proliferation and adhesion activities of circulating EPC,the level of NO from both plasma and EPC production were dramatically decreased in hypertensive urgency group( P < 0. 05). Furthermore,there was a strong correlation between FMD,NO( from both plasma and EPC production) and circulating EPC activities( migration,proliferation and adhesion activities).However,there were no significant differences among the level of GM-CSF,VEGF and IL-6 from both plasma and EPC production in two groups( P>0. 05). Conclusion The functional activities of circulating EPC were declined in hypertensive urgency,which is positively correlated with the level of NO and FMD.

关 键 词：高血压亚急症 内皮祖细胞 内皮功能 

分 类 号：R5[医药卫生—内科学]