替比夫定治疗的慢乙肝患者中Th17细胞释放IL-26的变化研究  

The dynamic change of IL-26 from Th17 cell of chronic hepatitis B patient treated with telbivudine

作　　者：薛雅方 蒋黎 周剑 张红玉[4] 袁吉钊 XUE Yafang;JIANG Li;ZHOU Jian;ZHANG hongyu;YUAN Jizhao(Department of Ultrasound,Southwestern Hospital,Army Medical University,Chongqing 400038,China;Department of Infectious Diseases,Southwestern Hospital,Army Medical University,Chongqing 400038,China;Institute of Immunology,Army Medical University,Chongqing 400038,China;Department of Emergency,Second Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]陆军军医大学西南医院超声科,重庆400038 [2]陆军军医大学西南医院感染病研究所重庆市重点实验室,重庆400038 [3]陆军军医大学基础医学院免疫学教研室,重庆400038 [4]重庆医科大学附属第二医院急诊科,400016

出　　处：《免疫学杂志》2020年第6期539-544,共6页Immunological Journal

基　　金：重庆市基础与前沿研究计划(cstc2015jcyjA10119);陆军军医大学基础医学院基金(2019JCZX10)。

摘　　要：目的通过检测替比夫定治疗的慢性乙型肝炎(chronic hepatitis B,CHB)患者中IL-26的动态变化及与其它炎症因子的相关性,揭示其在CHB疾病中可能的促炎作用。方法将51个CHB患者和28个健康人纳入本研究。收集患者在替比夫定(tebivudine,LdT)治疗期间(0、12、24、36和52周)外周血,分析HBsAg、HBeAg、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)和HBV病毒载量等临床数据。流式细胞仪分离CD4^+T、CD4^-T细胞,酶联免疫吸附检测血浆中IL-26、IL-17蛋白水平,荧光定量PCR检测IL-26、RORγt的mRNA水平。结果与健康对照组相比CHB患者血浆IL-26水平显著升高,LdT治疗期间IL-26水平呈时间依赖性下降。治疗期间血浆IL-26浓度与T辅助17(Th17)细胞分泌IL-17水平呈相似的下降趋势。CHB患者外周血单个核细胞中IL-26^+CD4^+细胞百分率明显高于IL-26^+CD4^-细胞百分率,CD4^+T细胞表达的IL-26和RORγt的mRNA水平呈正相关,且随治疗进程其表达水平逐渐降低,提示Th17细胞可能是IL-26主要源细胞。结论在CHB患者的外周血中,IL-26主要由Th17细胞产生,是一种潜在的CHB预后和治疗的生物标志物。The study was designed to detect the changes of IL-26 and other inflammatory factors in patients with chronic hepatitis B(CHB)treated with telbivudine(LdT),and to reveal the pro-inflammatory effect of IL-26 in CHB.Total of 51 CHB patients and 28 healthy individuals were recruited,and the peripheral blood was collected during LDT treatment period(0,12,24,36 and 52 weeks).The clinical data of HBsAg,HBeAg,ALT,AST and HBV load were analyzed;CD4^+T and CD4^-T cells were separated by flow cytometry;the levels of IL-26 and IL-17 proteins in plasma were detected by enzyme-linked immunosorbent assay;and the mRNA levels of IL-26 and RORγwere detected by quantitative PCR.Compared with the control group,the level of IL-26 in CHB patients was significantly higher,which decreased in a timedependent manner during LdT treatment which was similar to the change of IL-17.The percentage of IL-26^+CD4^+cells in PBMC of patients with CHB was significantly higher than that of IL-26^+CD4^-cells.The level of IL-26 expressed by CD4^+T cells was positively correlated with the level of RORγt mRNA,and the level of IL-26 expression decreased gradually with the treatment,suggesting that Th17 cells may be the main source cells of IL-26.Taken together,in the peripheral blood of CHB patients,IL-26 is mainly produced by Th17 cells,which is a potential biomarker for prognosis and treatment of CHB.

关 键 词：IL-26 替比夫定 慢性乙型肝炎 TH17 

分 类 号：R392.7[医药卫生—免疫学]