天麻胶囊HPLC指纹图谱的建立和聚类分析  

HPLC Fingerprint and Cluster Analysis of Tianma Capsules

作　　者：杨秀丽[1] 徐水琴 Yang Xiuli;Xu Shuiqin(Space Center Hospital,Beijing 100049,China)

机构地区：[1]航天中心医院,北京100049

出　　处：《中国药师》2020年第5期853-856,共4页China Pharmacist

摘　　要：目的:建立天麻胶囊的HPLC指纹图谱,并进行聚类分析。方法:采用HPLC法,色谱柱为Agilent ZORBAX Eclipse XDB-C18柱(250 mm×4.6 mm,5μm),以乙腈(A)-0.1%磷酸水溶液(B)为流动相,梯度洗脱,流速为1.0 ml·min-1,检测波长为265 nm,柱温为30℃,进样量为10μl。以天麻素为参照,绘制10批样品的HPLC指纹图谱;采用《中药色谱指纹图谱相似度评价系统》(2004年A版)进行相似度评价,确定共有峰;采用SPSS 22.0软件对10批样品进行聚类分析。结果:10批天麻胶囊指纹图谱标定了共有峰15个,指认了其中8个化学成分。10批样品指纹图谱与对照指纹图谱的相似度均大于0.997。聚类分析结果显示,10批样品可聚为3类,S1、S2、S6、S7聚为一类,S3、S4、S5、S10聚为一类,S8、S9聚为一类。结论:所建立的HPLC指纹图谱和聚类分析结果可为天麻胶囊的质量评价提供参考。Objective:To establish the HPLC fingerprint of Tianma capsules and to conduct the cluster analysis.Methods:An HPLC method was adopted.The determination was carried out on an Agilent ZORBAX Eclipse XDB-C18 column(250 mm×4.6 mm,5μm)with gradient elution by acetonitrile(A)-0.1%phosphoric acid solution(B)at a flow rate of 1.0 ml·min-1,and the detection wavelength was 265 nm,the column temperature was 30℃and the sample size was 10μl.Using gastrodin as the reference,HPLC fingerprints of ten batches of samples were determined.The similarity of 10 batches of samples was evaluated by TCM Chromatographic Fingerprint Similarity Evaluation System(2004 A edition)to confirm the common peaks.Cluster analysis was performed by using SPSS 22.0 software.Results:There were 15 common peaks in the fingerprints of the ten batches of Tianma capsules,and eight of which were identified.The similarities of fingerprints of the ten batches of Tianma capsules were over 0.997.Cluster analysis showed that the ten batches of samples were clustered into three categories.The first category was S1,S2,S6 and S7,the second was S3,S4,S5 and S10,and the third was S8 and S9.Conclusion:The established HPLC fingerprint and the results of cluster analysis can provide reference for the quality evaluation of Tianma capsules.

关 键 词：天麻胶囊 指纹图谱 高效液相色谱法 聚类分析 天麻素 

分 类 号：TQ460.7[化学工程—制药化工]