八珍益母丸中9个成分的含量测定及其主成分、聚类分析  

Content Determination of 9 Constituents in Bazhen Yimu Pills and Its Principal Component and Cluster Analysis

作　　者：平静[1] 南春红[1] 王思源[1] 岳志军[1] Ping Jing;Nan Chunhong;Wang Siyuan;Yue Zhijun(Viral Laboratory of Liaoning Traditional Chinese Medicine University Affiliated Hospital,Shenyang 110032,China)

机构地区：[1]辽宁中医药大学附属医院病毒实验室,沈阳110032

出　　处：《中国药师》2020年第5期857-861,共5页China Pharmacist

摘　　要：目的:建立同时测定八珍益母丸中9种成分含量的方法,同时对其进行主成分分析及聚类分析。方法:采用HPLC法,色谱柱Waters Sunfire C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-乙腈(35∶65,A)-0.5%磷酸溶液(B),梯度洗脱;流速:1.0 ml·min-1;检测波长:220 nm(白术内酯Ⅰ、白术内酯Ⅲ、茯苓酸)、230 nm(芍药苷、甘草苷)、280 nm(盐酸益母草碱、白术内酯Ⅱ)、320 nm(阿魏酸、毛蕊花糖苷);柱温:28℃;进样量:10μl。采用SPSS 22统计软件对含量测定结果进行主成分分析与聚类分析。结果:盐酸益母草碱、阿魏酸、毛蕊花糖苷、白术内酯Ⅰ、白术内酯Ⅲ、白术内酯Ⅱ、芍药苷、茯苓酸和甘草苷检测质量浓度线性范围分别为6.084~48.672,5.498~43.980,2.972~23.772,2.432~19.456,2.079~16.632,2.174~17.388,2.836~22.684,4.761~38.084,2.504~20.036μg·ml-1(r>0.9990);平均加样回收率为98.4%~100.2%,RSD为0.32%~0.88%(n=6);15批样品提取出2个主成分,聚类分析为3类。结论:本法简便、准确、重复性好,可以用于八珍益母丸的鉴定与评价,可作为八珍益母丸质量控制的重要科学依据之一。Objective:To establish a method for the simultaneous determination of 9 constituents in Bazhen Yimu pills,and to conduct principal component analysis and cluster analysis.Methods:An HPLC analysis was performed on a Waters Sunfire C18(250 mm×4.6 mm,5μm)column with the column temperature at 28℃and the sample size was 10μl.The mobile phase was methanol-acetonitrile(35∶65,A)-0.5%phosphoric acid solution(B)with gradient elution at the flow rate of 1.0 ml·min-1,and the detection wavelength was 220 nm for atractylenolideⅠ,atractylenolideⅢand pachymic acid,230 nm for paeoniflor and liquiritin,280 nm for leonurine hydrochloride and atractylenolideⅡ,and 320 nm for ferulic acid and acteoside.The principal component analysis and cluster analysis were conducted for the results of content determination by SPSS 22 statistical software.Results:The linear range was 6.084-48.672μg·ml-1 for leonurine hydrochloride,5.498-43.980μg·ml-1 for ferulic acid,2.972-23.772μg·ml-1 for acteoside,2.079-16.632μg·ml-1 for atractylenolideⅠ,2.174-17.388μg·ml-1 for atractylenolideⅢ,2.504-20.036μg·ml-1 for atractylenolideⅡ,2.836-22.684μg·ml-1 for paeoniflor,4.761-38.084μg·ml-1 for pachymic acid and 2.504-20.036μg·ml-1 for liquiritin(r>0.9990).The average recoveries were 98.4%-100.2%,RSDs were 0.32%-0.88%(n=6).Two main components belonged to the samples of the 15 batches of Bazhen Yimu pills.The samples of 15 batches of Bazhen Yimu pills could be divided into 3 categories.Conclusion:The method is simple and accurate with good repeatability,which can be used for the identification and evaluation of Bazhen Yimu pills,and provides important scientific basis for the quality control of Bazhen Yimu pills.

关 键 词：八珍益母丸 高效液相色谱法 含量测定 聚类分析 主成分分析 

分 类 号：R927.2[医药卫生—药学]