两种灭活方法对2019新型冠状病毒咽拭子标本病毒核酸检测结果的影响  

The effect of 2 different inactivation methods on the results of viral nucleic acid in 2019 new coronavirus throat swab specimens

作　　者：姜蕾[1] 张丽媛[2] 刘大宁[2] JIANG Lei;ZHANG Liyuan;LIU Daning(Laboratory of Puyang Oilfield General Hospital,Puyang,Henan,China,457001;Laboratory of Puyang people's Hospital,Puyang,Henan,China,457000)

机构地区：[1]濮阳市油田总医院检验科,河南濮阳457001 [2]濮阳市人民医院检验科,河南濮阳457000

出　　处：《分子诊断与治疗杂志》2020年第4期410-413,共4页Journal of Molecular Diagnostics and Therapy

摘　　要：目的探讨2019新型冠状病毒(2019⁃nCoV)核酸提取前,两种不同灭活方法分别处理咽拭子标本,对于荧光定量PCR检测结果的影响。方法将3例2019新型冠状病毒阳性标本各采用两种不同灭活方法处理,未灭活,56℃灭活30 min和加入胍盐RNA保存液灭活3组样本分别用手工离心柱法提取核酸,采用两种不同核酸检测试剂同时检测病毒含量,对qPCR结果进行统计学分析。结果两种不同核酸检测试剂结果显示,未灭活、56℃灭活30 min和加入胍盐RNA保存液灭活3组结果相关性明显,三组之间相关系数均在0.98以上(P<0.001)。未灭活、56℃灭活30 min和加入胍盐RNA保存液灭活三组结果差异均在1以下,具有较好的一致性。结论56℃灭活30 min和胍盐RNA保存液灭活标本,对2019新型冠状病毒核酸荧光定量PCR结果无影响。Objective To investigate the effect of two different pharyngeal swab specimens inactivation methods on the results of 2019 new coronary virus fluorescent quantitative PCR.Method Three cases of 2019 new coronary virus positive specimens were treated by two different inactivation methods,the nucleic acids were extracted by manual centrifugation method from three groups of samples,which were inactivated at 56℃for 30 minutes and inactivated with guanidine salt RNA preservation solution.Simultaneous detection of virus content with two different nucleic acid detection kits,the results of qPCR were analyzed statistically.Result The results of two different nucleic acid detection reagents showed that,the results of the three groups,which were not inactivated,inactivated at 56℃for 30 minutes and inactivated with guanidine salt RNA preservation solution,were significantly correlated and had good consistency.The correlation coefficients among the three groups were all above 0.98(P<0.001).Conclusion 2019 new coronary virus nucleic acid qPCR results were not affected by the two inactivation methods.

关 键 词：2019新型冠状病毒 新型冠状病毒肺炎 荧光定量PCR检测 

分 类 号：R563.1[医药卫生—呼吸系统] R440[医药卫生—内科学]