血管内皮生长因子基因936C/T位点多态性与急性心肌梗死的关系  

Correlation between 936C/T site polymorphisms of vascular endothelial growth factor gene and acute myocardial infarction

作　　者：常亚伟 李赛 琚绍坦 CHANG Yawei;LI Sai;JU Shaotan(Department of cardiovascular medicine,Henan provincial third people's hospital,Zhengzhou,Henan,Chi⁃na,450000)

机构地区：[1]河南省直第三人民医院心血管内科,河南郑州450000

出　　处：《分子诊断与治疗杂志》2020年第2期151-155,共5页Journal of Molecular Diagnostics and Therapy

摘　　要：目的研究血管内皮生长因子(VEGF)936C/T多态性与急性心肌梗死(AMI)的关系。方法纳入2016年7月至2018年12月在本院住院且首次诊断为AMI的120例患者作为AMI组及同期进行健康体检的150例志愿者作为对照组。提取外周血基因组DNA,通过PCR反应、酶切反应以及琼脂糖凝胶电泳检测VEGF基因936C/T位点多态性。结果AMI组VEGF基因936C/T位点的基因型频率(CC:34.17%,CT+TT:65.83%)与对照组的基因型频率(CC:54.67%,CT+TT:45.33%)差异有统计学意义(P=0.001);AMI组VEGF基因936C/T位点等位基因频率(C:59.58%;T:40.42%)与对照组等位基因频率(C:73.67%;T:26.33%)差异有统计学意义(P=0.001):AMI组患者VEGF基因936C/T位点CT+TT基因型比例、等位基因T频率明显高于对照组(P<0.05)。AMI组中VEGF基因936C/T位点CT+TT基因型患者的血清CK⁃MB峰值、cTnI峰值明显高于CC基因型患者,血清VEGF水平明显低于CC基因型患者(P<0.05)。Logistic回归分析显示,年龄、吸烟、高血压、糖尿病、高脂血症、VEGF基因CT+TT基因型是AMI发生的危险因素。结论VEGF基因936C/T多态性与AMI的发生有关,其中等位基因T可能是AMI发生的易感等位基因。Objective To investigate the correlation between vascular endothelial growth factor(VEGF)936C/T polymorphism and acute myocardial infarction(AMI).Methods 120 cases of patients hos⁃pitalized and diagnosed with AMI for the first time in our hospital from July 2016 to December 2018 were en⁃rolled as the AMI group and 150 volunteers who had physical examination in the same period were enrolled as the control group.Genomic DNA was extracted from peripheral blood,and the polymorphism of VEGF gene 936C/T site was detected by PCR,enzyme digestion and agarose gel electrophoresis.Results The genotype frequency of VEGF gene 936C/T site in the AMI group(CC:34.17%,CT+TT:65.83%)was significantly different from that of the control group(CC:54.67%,CT+TT:45.33%)(P=0.001).Allele frequency of VEGF gene 936C/T site in the AMI group(C:59.58%;T:40.42%)had statistical significance with control group allele frequency(C:73.67%;T:26.33%)(P=0.001).In the AMI group,the proportion of CT+TT genotype and the frequency of T allele at 936C/T of VEGF gene were significantly higher than those of the con⁃trol group(P<0.05).The CK⁃MB peak,cTnI peak of the patients with CT+TT genotype at 936C/T site of VEGF gene in AMI group were significantly higher than those of the patients with CC genotype,and the level of VEGF were significantly lower than those of the patients with CC genotype(P<0.05).Logistic regression analysis showed that age,smoking,hypertension,diabetes,hyperlipidemia,VEGF gene CT+TT genotype were risk factors for AMI.Conclusion The 936C/T polymorphism of VEGF gene is related to the develop⁃ment of AMI,and the allele T may be the susceptible allele of AMI.

关 键 词：急性心肌梗死 血管内皮生长因子 基因多态性 危险因素 

分 类 号：R542.22[医药卫生—心血管疾病]