纳米铟锡氧化物对大鼠肺损伤及氧化应激的影响  

Effects of nano-indium tin oxide on lung injury and oxidative stress in rats

作　　者：周春玲 陈蕾 蔚岩 曹福源 李清钊 荆男 肖经纬[4] 刘楠 关维俊 李斌[4] ZHOU Chun-ling;CHEN Lei;YU Yan;CAO Fu-yuan;LI Qing-zhao;JING Nan;XIAO Jing-wei;LIU Nan;GUAN Wei-jun;LI Bin(School of Public Health,North China University of Technology Hebei Provincial Coal Mine Health and Safety Laboratory,Tangshan 063000,China)

机构地区：[1]华北理工大学公共卫生学院河北省煤矿卫生与安全实验室,河北唐山063000 [2]华北理工大学实验动物中心,河北唐山063000 [3]华北理工大学冀唐学院,河北唐山063000 [4]中国疾病预防控制中心职业卫生与中毒控制所,北京100050

出　　处：《工业卫生与职业病》2020年第3期177-181,共5页Industrial Health and Occupational Diseases

基　　金：国家公益性卫生行业科研专项(201402021)。

摘　　要：目的探讨非暴露式气管灌注纳米铟锡氧化物对SD大鼠肺组织的损伤作用。方法选取6~8周龄SPF级SD雄性大鼠40只,体质量为(200±10)g,随机分为对照组、低剂量组(1.2mg/kg)、中剂量组(3mg/kg)、高剂量组(6mg/kg)。常规饲养1周,给予非暴露式气管灌注染毒,每周2次,间隔3d,连续染毒12周。染毒期间每周称量体质量,观察体质量变化。染毒结束后腹腔注射水合氯醛麻醉处死,留取肺组织、血清和灌洗液。采用试剂盒测定灌洗液中总超氧化物歧化酶(T-SOD)、丙二醛(MDA)、乳酸脱氢酶(LDH)和血清中的谷胱甘肽过氧化物酶(GSH-Px),采用酶联免疫吸附法(ELISA)测定肺组织中的血红素氧合酶-1(HO-1)含量和血清中肿瘤坏死因子(TNF-α)的变化,采用免疫组化法检测肺组织中TNF-α蛋白表达水平,制作HE染色切片,观察各组肺组织病理变化。结果染毒期间,大鼠一般情况无明显异常,体质量稳定增长;病理切片显示,大鼠在灌注纳米铟锡氧化物后,各染毒组肺组织都呈现不同程度的损伤,以高剂量组最为明显。与对照组比较,各剂量染毒组肺灌洗液中MDA含量、T-SOD活力均明显上升(P<0.05),中、高剂量染毒组LDH活力明显上升(P<0.05),各剂量染毒组大鼠肺组织和血清中TNF-α蛋白表达水平均明显降低(P<0.05),各剂量染毒组肺组织中的HO-1含量明显下降(P<0.05),GSH-Px水平无明显差异(P>0.05)。结论非暴露式气管灌注纳米铟锡氧化物能导致SD大鼠肺氧化损伤,发生病理损害及功能障碍,破坏了机体氧化/抗氧化的平衡。Objective Purpose To investigate the effect of nano-indium tin oxide on lung tissue injury in SD rats by non-exposed trachea infusion.Methods 40SPF SD male rats aged 6-8weeks with a body weight of(200±10)g were randomly divided into control group,low dose group(1.2mg/kg),medium dose group(3mg/kg)and high dose group(6mg/kg).The rats were routinely reared for 1week and given non-exposure tracheal infusion,twice a week,3days apart,for 12weeks of continuous exposure.Rat body weight was weighed weekly during the exposure period,and the body weight changes were observed.After the end of the poisoning,the rats anesthetized with chloral hydrate and then sacrificed,and their lung tissues,serum and lavage fluid were taken.The kit measured total superoxide dismutase(T-SOD),malondialdehyde(MDA),lactate dehydrogenase(LDH)and glutathione peroxidase(GSH-Px)in serum in lavage fluid.Determination of heme oxygenase(HO-1)content in serum and tumor necrosis factor(TNF-α)in serum by enzyme-linked immunosorbent assay(ELISA).Immunohistochemical method was used to detect the changes of TNF-αprotein expression level in lung tissue.Results During the exposure period,the general condition of the rats was no obvious abnormality,and the weight of the rats steadily increased.Pathological sections showed that after infusion of nano-indium tin oxide,the lung tissue of each exposed group showed different degrees of damage,which was most obvious in the high dose group.Compared with the control group,the MDA content and TSOD activity in the lung lavage fluid of each dose group increased significantly(P<0.05),and the LDH activity of the medium and high dose groups increased significantly(P<0.05).The expression of TNF-αprotein in lung tissue and serum of the rats in each dose group was significantly lower(P<0.05)than the control group.The HO-1content in the lung tissue of each dose group was significantly decreased(P<0.05).There was no significant difference in GSH-Px levels between the groups(P>0.05)compared to the control group.Conclusions Non

关 键 词：纳米铟锡氧化物 亚慢性肺损伤 氧化应激 

分 类 号：R135.2[医药卫生—劳动卫生]