大豆γ-生育酚的混合遗传分析与QTL定位  

Mixed Inheritance Analysis and QTL Mapping for γ-Tocopherol Content in Soybean

作　　者：梁慧珍[1,2] 许兰杰[1] 董薇[1] 余永亮[1] 杨红旗[1] 谭政委 李磊 刘新梅 LIANG HuiZhen;XU LanJie;DONG Wei;YU YongLiang;YANG HongQi;TAN ZhengWei;LI Lei;LIU XinMei(Sesame Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002;Xixia Branch,Henan Academy of Agricultural Sciences,Xixia 474550,Henan)

机构地区：[1]河南省农业科学院芝麻研究中心,郑州450002 [2]河南省农业科学院西峡分院,河南西峡474550

出　　处：《中国农业科学》2020年第11期2149-2160,共12页Scientia Agricultura Sinica

基　　金：国家现代农业产业技术体系建设专项(CARS-21);国家农业科研杰出人才及其创新团队(农财发(2016)45号);国家973计划(2008CB117005);河南省药用植物遗传改良创新型科技团队、河南省科技攻关计划(182102310062);河南省重大科技专项(181100110300);河南省农业科学院创新创意(2020CX03)。

摘　　要：【目的】通过对大豆γ-生育酚进行混合遗传和QTL定位分析,了解其遗传机制,定位其主效QTL,为高γ-生育酚含量大豆品种的选育奠定基础。【方法】以栽培大豆晋豆23为母本,以山西农家品种大豆灰布支黑豆为父本杂交衍生的重组自交系作为供试群体构建遗传图谱。图谱全长2047.6 cM,平均图距8.8 cM,包括227个SSR标记,232个标记位点。重组自交系试验群体及亲本材料分别于2011年、2012年和2015年夏季在河南省农业科学院原阳试验基地种植,冬季在海南省三亚南繁试验基地种植。田间试验采取随机区组设计,2次重复。从6个环境中每个家系选取15.00 g籽粒饱满,大小一致的大豆种子,利用高效液相色谱法定性、定量测定样品中的γ-生育酚含量。采用主基因+多基因混合遗传分离分析法,对大豆γ-生育酚含量进行混合遗传分析;采用WinQTLCart 2.5复合区间作图法,对大豆γ-生育酚含量进行QTL定位分析。【结果】主基因+多基因混合遗传分析表明,γ-生育酚受2对重叠作用主基因×加性多基因控制。遗传基因分布在双亲中。三亚试验数据检测出2对主基因间上位性效应值为0.4010—0.5169和多基因的加性效应值为0.1797—0.2146,主基因遗传率为11.27%—13.05%,多基因遗传率为82.51%—86.55%,多基因效应大于主基因效应。原阳试验数据检测到2对主基因间上位性效应值为0.9646—1.8455,主基因遗传率为39.51%—58.96%,没有检测出多基因效应。采用WinQTLCart 2.5复合区间作图(CIM)共检测到9个影响γ-生育酚的QTL,分布于A1(Chr.5)、A2(Chr.8)、C1(Chr.4)、K(Chr.9)、M(Chr.7)和G(Chr.8)6条染色体中,单个QTL的贡献率为7.29%—29.55%。qγ-G-1同时在2011年原阳、2012年三亚、2015年三亚3个环境下检测到,且均定位在G(Chr.18)染色体Satt275—Satt038标记区间0.01 cM处,解释的表型变异分别为8.97%、8.12%和7.91%。qγ-A1-1同时在2011年原阳和2015年原阳2个【Objective】Inheritance and main QTL for the content ofγ-tocopherol were detected by genetic analysis and QTL mapping.The results lay a genetic foundation for the selection of soybean varieties with highγ-tocopherol content in soybean.【Method】The RILs were derived from a cross between Jindou23 of commercial cultivar as the female parent and Huibuzhi of farm variety from Shanxi Province as the male parent that construct genetic linkage map.The map consisting of 232 marker loci spanned a total of 2047.6 cM in length with an average spacing of 8.8 cM between adjacent marker loci.The parent lines and the RILs were cultivated in summer at Yuanyang experimental farm of Henan Academy of Agricultural Sciences,and in winter at Sanya of Hainan province in 2011,2012,2015.Random block design was adopted in field experiment,and the entire planting experiment was replicated twice.15.00 g fully filled and uniform soybean seeds from each RILs in six environments were sampled.The content ofγ-tocopherol was quantitatively and qualitatively analysis by High Performance Liquid Chromatography(HPLC).The content ofγ-tocopherol in soybean was analyzed by major gene plus polygene mixed inheritance model approach.QTL for the content ofγ-tocopherol in soybean were detected by composite interval mapping model using WinQTLCart 2.5.【Result】The results showed that the content ofγ-tocopherol was controlled by two pairs of main overlapping major gene×additive polygenes using major gene plus polygene mixed inheritance analysis.According to the data of Sanya,the epistasis effect value between two major genes was 0.4010-0.5169,and the additive effect value of polygene was 0.1797-0.2146.The results of Sanya experiment showed that the heritability of major gene and polygene were 11.27%-13.05%and 82.51%-86.55%,respectively.The polygene effect was greater than that of major gene effect.The data of Yuanyang experiment showed that the epistasis effect between the two major genes was 0.9646-1.8455,and the heritability of the major genes

关 键 词：大豆 γ-生育酚 遗传 主基因+多基因 基因定位 上位性 

分 类 号：S565.1[农业科学—作物学]