Colloidal(Gd0.98Nd0.02)2O3 nanothermometers operating in a cell culture medium within the first and second biological windows  

作　　者：M.L.Debasu H.Oliveira J.Rocha L.D.Carlos 

机构地区：[1]Department of Physics,CICECO-Aveiro Institute of Materils,Universidade de Aveiro,3810-193,Aveiro,Portugal [2]Department of Chemistry,CICECO-Aveiro Institute of Materials,Universidade de Aveiro,3810-193,Aveiro,Portugal [3]Department of Biology,CESAM,Laboratory of Biotechnology and Cytomics,Universidade de Aveiro,3810-193,Aveiro,Portugal

出　　处：《Journal of Rare Earths》2020年第5期483-491,共9页稀土学报（英文版）

基　　金：Project supported by CICECO-Aveiro Institute of Materials(FCT Ref.UID/CTM/50011/2019);CESAM(Ref No.FCT UID/AMB/50017-POCI-01-0145-FEDER-007638)。

摘　　要：Non-contact,self-referenced and near-infrared luminescent nanothermometers have been recognized as emerging tools in the fields of nanomedicine and nanotechnology due to their great capability of precise temperature readout at the nanoscale and real-time deep-tissue imaging.However,the development of multifunctional and biocompatible luminescent nanothermometers operating within the optically transparent biological windows with high thermal sensitivity(>2.0%/K)remains challenging.Here,we present(Gd0.98Nd0.02)2O3 nanothermometers operated effectively within the first and second biological windows upon continuous-wave laser diode excitation at 808 nm.Ratiometric thermometric parameters are defined by the relative changes in the emission intensities originating from the two Stark components of the 4 F3/2 level(R2 and R1)to the 4 I9/2(900-1000 nm),4 I11/2(1035-1155 nm)and 4 I13/2(1300-1450 nm)multiplets.The thermo metric parameters are evaluated for colloidal samples in a cell culture medium and powder samples,and the highest thermal sensitivity(2.18%/K at 298 K)is attained for the former in the first biological window(both the excitation and emission in the 800-965 nm range).The repeatability and temperature uncertainty are 99%and 1.2 K,respectively.The nanothermometers are biocompatible with human MNT-1 melanoma and HaCaT cells for 24 h of exposure and nanoparticle concentration up to 0.400 mg/mL,showing their potential for applications in nanomedicine,e.g.,intracellular imaging and temperature mapping.

关 键 词：GD2O3 Nd3^+-doped nanoparticles Luminescent nanothermometry Thermal sensitivity Cytotoxicity of nanoparticles Rare earths 

分 类 号：TB383.1[一般工业技术—材料科学与工程] TH79[机械工程—仪器科学与技术]