凡口铅锌矿酸性底泥可培养微生物资源的探索分离  

Isolation of Culturable Microbes in Sediments from Fankou Lead-Zinc Mine

作　　者：房保柱[1] 王怡欢 张堃 廖俊杰[2] 欧阳欣[2] 岳秀 肖吉 李文均[1] FANG Bao-zhu;WANG Yi-huan;ZHANG Kun;LIAO Jun-jie;OUYANG Xin;YUE Xiu;XIAO Ji;LI Wen-jun(Schl.of Life Sci.,Sun Yat-jsen Uni.,Guangzhou 510275;Schl.of Eco-jEnviron′t Tech.,Guangdong Light Indust.Vocational Polytech.,Guangzhou 510300)

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]广东轻工职业技术学院生态环境技术学院,广东广州510300

出　　处：《微生物学杂志》2020年第2期10-21,共12页Journal of Microbiology

基　　金：广东省自然科学基金项目(2016A030312003,2018A0303130014);广东轻工职业技术学院优秀青年基金项目(QN2018-003)。

摘　　要：通过改良优化多种传统分离培养基,对凡口铅锌尾矿的酸性底泥可培养微生物资源进行分离,并结合多种分子标记基因对分离资源进行系统发育地位分析。结果表明,凡口铅锌尾底泥中分离获得细菌41株,分属于放线菌门(Actinomycetes)、变形菌门(Proteobacteria)和厚壁菌门(Firmicutes)的11目,18科,18属;真菌8株,分属于2门,5目,5科,5属;藻类1株,属于Galdieria属的红藻。改良ISP 2和改良9号培养基的分离效果较好,分离获得的微生物多样性较为良好。研究结果为矿山酸性废水微生物多样性研究提供参考,为酸性废水的修复及生态治理研究提供物质基础。Adopting improved and optimized the traditional various isolation media,microbes from tail bottom mud in Fankou lead-zinc mine were preformed the isolation of culturable microbial resources,combined with different molecular biomarker genes to carry out phylogenetic position analysis.The results showed that a total of 41 bacterial strains obtained from Fankou lead-zinc tail bottom mud respectively belonged to 11 orders,18 families and 18 genera of the phyla of Actinomycetes,Proteobacteria,and Firmicutes;and 8 fungal strains respectively belonged to 2 phyla,5 orders,5 families,and 5 genera;1 alga strain belonged to the genus Galdieria of the phylum of red alga.The improved ISP 2 and improved No.9 medium have shown better isolation effect,and the isolated-and-obtained had fine microbial diversity.This study had laid theoretical basis for studying on the microbial diversity in acid wastewater of mine,and provided material foundation for restoring acid wastewater and ecological control research.

关 键 词：凡口铅锌尾矿 极端微生物 分离 

分 类 号：Q93-33[生物学—微生物学]