In vitro testicular organogenesis from human fetal gonads produces fertilization-competent spermatids  

作　　者：Yan Yuan Laihua Li Qing Cheng Feiyang Diao Qiao Zeng Xiaoyu Yang Yibo Wu Hao Zhang Mingqian Huang Junqing Chen Quan Zhou Yunfei Zhu Rong Hua Jianyu Tian Xin Wang Zuomin Zhou Jie Hao Jinjin Yu Dong Hua Jiayin Liu Xuejiang Guo Qi Zhou Jiahao Sha 

机构地区：[1]Human Reproductive and Genetic Center,Affiliated Hospital of Jiangnan University,214062 Wuxi,Jiangsu,China [2]State Key Laboratory of Reproductive Medicine,Department of Histology and Embryology,Nanjing Medical University,211166 Nanjing,Jiangsu,China [3]State Key Laboratory of Reproductive Medicine,Womens Hospital of Nanjing Medical University,Nanjing Medical University,210029 Nanjing,Jiangsu,China [4]State Key Laboratory of Reproductive Medicine,Clinical Center of Reproductive Medicine,First Affiliated Hospital of Nanjing Medical University,Nanjing Medical University,210029 Nanjing,Jiangsu,China [5]State Key Laboratory of Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,100101 Beijing,China

出　　处：《Cell Research》2020年第3期244-255,共12页细胞研究（英文版）

基　　金：supported by National Key R&D Program(2017YFA0103803);National Natural Science Foundation of China(31890784);National Key R&D Program(2016YFA0500903,2016YFA0503300).

摘　　要：Unlike most organs that mature during the fetal period,the male reproductive system reaches maturity only at puberty with the commencement of spermatogenesis.Robust modelling of human testicular organogenesis in vitro would facilitate research into mechanisms of and factors affecting human spermatogenic failure and male fertility preservation in prepubertal tumor patients.Here,we report successful recapitulation of human testicular organogenesis in vitro from fetal gonadal ridge.Our model displayed the formation of mature seminiferous epithelium and self-renewing spermatogonia.Remarkably,in vitro-derived haploid spermatids have undergone meiotic recombination,and showed increased genetic diversity as indicated by genetic analysis.Moreover,these spermatids were able to fertilize oocytes and support subsequent blastocyst formation.The in vitro testicular organogenesis system described here will play an important role in elucidating the regulation of human testis development and maintaining male fertility in prepubertal cancer patients.

关 键 词：FETAL ORGANS fertility 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]