利用RNA干涉研究水稻OsROSES1基因的功能  

Function Analysis of a Reduced Organ Size with Early Senescence Gene(OsROSES1)by RNA Interference

作　　者：龚晓平 王楠[2] 况晓明 曾正明[3] 张长伟[2] 李加胜 刘勤 张致力 何光华[2] 罗挺 GONG Xiao-ping;WANG Nan;KUANG Xiao-ming;ZENG Zheng-ming;ZHANG Chang-wei;LI Jia-sheng;LIU Qin;ZHANG Zhi-li;HE Guang-hua;LUO Ting(Yudongnan Academy of Agricultural Sciences,Fuling Chongqing 408000,China;Rice Research Institute,Southwest University/Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops,Chongqing 400715,China;Institute of Rice and Sorghum,Sichuan Academy of Agricultural Sciences,Deyang Sichuan 618000,China)

机构地区：[1]重庆市渝东南农业科学院,重庆涪陵408000 [2]西南大学水稻研究所/转基因植物与安全控制重庆市重点实验室,重庆400715 [3]四川省农业科学院水稻高粱研究所,四川德阳618000

出　　处：《西南大学学报（自然科学版）》2020年第6期1-10,共10页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市(重点)基础科学与前沿技术研究项目(cstc2015jcyjBX0109);重庆市基础研究与前沿探索项目(cstc2018jcyjAX0447).

摘　　要：生物体最终的大小或者生物体个体器官的大小都受细胞增殖、扩张等因素的调控.通过RNA干涉对水稻器官大小变小且伴随叶片早衰基因OsROSES1的功能进行研究,实时定量PCR分析结果显示,干涉转基因植株OsROSES1基因表达水平显著下调.与野生型相比,干涉转基因植株OsROSES1表达水平下调的转基因株系整体变矮,叶片卷曲且明显变短变窄,叶片横切面维管束数目减少,表明OsROSES1基因的下调引起细胞数量的减少和细胞大小减小导致水稻器官大小明显减小,同时,叶片气孔密度和开度的增加导致叶片提前衰老.结果表明,ROSES1可作为水稻BEL1-Like"同源盒基因"在植物器官发育过程中通过调控细胞增殖和扩张从而控制器官大小和光合作用.The ultimate size of an organism and its organs is regulated by cell proliferation and cell expansion.The function of a“reduced organ size with early senescence gene”,OsROSES1,was analyzed by RNA interference in this study.The results of semi-quantitative RT-PCR and quantitative PCR analyses indicated that OsROSES1 expression levels were significantly downregulated in the transgenic plants.Compared with the wild-type plants,the OsROSES1-RNAi transgenic lines had reduced plant height,shorter and narrower curled leaves and fewer vascular bundles.Phenotypic analysis of the OsROSES1-RNAi transgenic lines demonstrated that the drastic reduction in organ size was attributed to decreased cell number and cell size,whereas the early leaf senescence probably resulted from increased stomatal density and aperture.The above results suggested that ROSES1 acts and coordinates with WUS or other homeobox genes to regulate meristem maintenance involved in controlling organ size and photosynthesis by regulating both cell proliferation and cell expansion.

关 键 词：水稻 器官大小 RNA干涉 气孔密度 叶片衰老 

分 类 号：S511[农业科学—作物学]