Increasing fidelity and efficiency by modifying cytidine base-editing systems in rice  

作　　者：Ruiying Qin Shengxiang Liao Juan Li Hao Li Xiaoshuang Liu Jianbo Yang Pengcheng Wei 

机构地区：[1]Key Laboratory of Rice Genetics&Breeding of Anhui Province,Institute of Rice Research,Anhui Academy of Agricultural Science,Hefei 230031,Anhui,China

出　　处：《The Crop Journal》2020年第3期396-402,共7页作物学报（英文版）

基　　金：funded by the Genetically Modified Breeding Major Project(2016ZX08010-002-008);the National Natural Science Foundation of China(31701405);the Natural Science Foundation of Anhui Province,China(1708085QC60)。

摘　　要：The efficiency of plant cytidine base-editing systems is limited, and unwanted mutations frequently occur in transgenic plants. We increased the cytidine editing frequency and fidelity of the plant base editor 3(BE3) and targeted activation-induced cytidine deaminase(CDA)(target-AID) systems by coexpressing three copies of free uracil–DNA glycosylase(UDG) inhibitor(UGI). The editing efficiency of the improved BE3 and CDA systems reached as high as 88.9% and 85.7%, respectively, in regenerated rice plants, with a very low frequency of unwanted mutations. The low editing frequency of the BE3 system in the GC context could be overcome by the modified CDA system. These results provide a highfidelity and high-efficiency solution for rice genomic base editing.

关 键 词：CRISPR-Cas9 Base editing BE3 CDA Oryza sativa 

分 类 号：S511[农业科学—作物学]