基于阵列芯片现场高灵敏同步快速检测粮油中氨基甲酸酯类农药残留混合污染  

On-site rapid sensitive and simultaneous detection for multiple carbamate pesticide residues in grains and oils based on microarray

作　　者：周丹 闵亚杰 戚欣 宁佳囡 吴文琴 张兆威[1,4,5] 李培武 ZHOU Dan;MIN Ya-jie;QI Xin;NING Jia-nan;WU Wen-qin;ZHANG Zhao-wei;LI Pei-wu(Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China;School of Life Sciences,Hubei University,Wuhan 430062,China;Luoyang Zhongke Chip Biotechnology Co.,Ltd.,Luoyang 471003,China;Laboratory of Quality and Safety Risk Assessment for Oilseed Products(Wuhan),Ministry of Agri⁃culture and Rural Affairs,Wuhan 430062,China;Quality Inspection and Test Center for Oilseed Products,Minis⁃try of Agriculture and Rural Affairs,Wuhan 430062,China)

机构地区：[1]中国农业科学院油料作物研究所,湖北武汉430062 [2]湖北大学生命科学学院,湖北武汉430062 [3]洛阳中科生物芯片技术有限公司,河南洛阳471003 [4]农业农村部油料作物风险评估重点实验室(武汉),湖北武汉430062 [5]农业农村部油料及制品质量监督检验测试中心,湖北武汉430062

出　　处：《中国油料作物学报》2020年第3期341-349,共9页Chinese Journal of Oil Crop Sciences

基　　金：国家重点研发计划(2018YFC1602501);中国农业科学院农业科技创新工程(CAAS-ZDRW202011)。

摘　　要：农产品质量安全是国家乡村振兴战略的重点和难点。氨基甲酸酯类农药残留严重威胁粮油产品质量安全与人民生命健康。目前,常用的氨基甲酸酯类农残检测方法主要是仪器检测法,无法满足现场对粮油农残混合污染进行现场高灵敏同步快速检测的需求。本研究在前期自主选育的克百威和甲萘威单克隆抗体基础上,研究并制备出农残混合污染同步快速检测阵列芯片,建立了一种现场高灵敏同步快速检测技术,实现了在30 min内同步定量克百威和甲萘威。经过优化,克百威和甲萘威的检测限分别为0.11、0.09 ng/g,线性范围为0.33~3000 ng/g、0.18~1500 ng/g。在花生样品检测中,克百威和甲萘威的加标回收率分别为88.4%~98.3%和90.1%~118.8%;批内差(RSD)分别为9.0%和3.5%;批间差(RSD)分别为12.3%和16.3%。该阵列芯片检测结果与液相色谱-质谱联用(HPLC-MS/MS)检测结果高度一致。该阵列芯片满足了对农产品中克百威和甲萘威进行现场高灵敏同步快速检测的需求,并可广泛应用于农产品质量安全、食品安全以及环境监测等领域。The quality and safety of agricultural products is the technique focus of the strategy of rural vitaliza⁃tion.Carbamate pesticide residues are seriously threatening the safety of agricultural product and human life and health.Nowadays,the common detection methods are instrument-based for carbamate pesticide residue,which dis⁃satisfy the request of an on-site rapid,sensitive and simultaneous detection for multiple carbamate pesticide resi⁃dues.Based on the home-made monoclonal antibodies against carbofuran and carbaryl respectivity,a microarray for rapid and simultaneous detection of multiple carbamate pesticide residues was developed within 30 min.Under the optimized conditions,the limits of detection(LODs)were found to be 0.11 and 0.09 ng/g,while the linear ranges were 0.33-3000 and 0.18-1500 ng/g for carbofuran and carbaryl respectively.By using spiked peanut samples,it recorded recoveries of 88.4%-98.3%,90.1%-118.8%for carbofuran and carbaryl respectively.The RSD of intraarray were found at 9.0%,3.5%,while the RSD of inter-array were recorded at 12.3%,16.3%respectively.The de⁃tection results of the microarray were highly consistent with that of HPLC-MS/MS.This proposed microarray provid⁃ed an on-site high-sensitivity,simultaneous and rapid detection of carbofuran and carbaryl,and could be widely used in the fields of agricultural product quality safety,food safety and environmental monitoring.

关 键 词：农药残留 氨基甲酸酯 阵列芯片 混合污染同步快速检测技术 农产品质量安全 

分 类 号：S132[农业科学—农业基础科学]