花生1,3,4-三磷酸肌醇5/6激酶ITPK家族基因的鉴定和分析  

Genome-wide identification and analysis of inositol 1,3,4-trisphosphate 5/6-kinase gene family in peanut

作　　者：任婧瑶 蒋春姬[1] 李新林 张鹤[1] 于海秋[1] 金华 REN Jing-yao;JIANG Chun-Ji;LI Xin-lin;ZHANG He;YU Hai-qiu;JIN Hua(Shenyang Agricultural University,Shenyang 110161,China;Dalian Nationalities University,Dalian 116600,China)

机构地区：[1]沈阳农业大学,辽宁沈阳110161 [2]大连民族大学,辽宁大连116600

出　　处：《中国油料作物学报》2020年第3期432-440,共9页Chinese Journal of Oil Crop Sciences

基　　金：现代农业产业技术体系建设专项(CARS-13);辽宁省博士启动基金(201601108)。

摘　　要：1,3,4-三磷酸肌醇5/6-激酶(ITPK)是一种保守的多功能酶,调控磷酸肌醇的代谢过程,广泛存在于动植物和线虫中。本研究从两个野生种花生基因组(Arachis duranensis和Arachis ipaensis)中获得Ad ITPK家族基因7个,Ai ITPK家族基因7个,利用生物信息学手段,系统地分析花生ITPK家族基因生物学特征。结果表明,Ad ITPKs和AiITPKs基因的染色体定位相似,在03号和05号染色体上都分别有2个ITPK家族成员,Ad ITPKs在A01、A08和A10号染色体上各1个,Ai ITPKs在B01、B07和B10号染色体上各1个;花生各ITPK基因含外显子数量在1~10个不等,可编码220~483个氨基酸;进化关系分析显示花生ITPK家族基因可分为3个亚家族;基于保守结构域分析显示,此家族蛋白含有4~6个保守结构基序。两基因组同源基因的二级结构相似性较大,而Ad ITPK5和Ai ITPK5、Ad ITPK6和AiITPK6两对同源基因例外;大部分基因的三级结构皆相似,但Ad ITPK1、Ad ITPK6、Ai ITPK1和Ai ITPK6与其余基因明显不同;花生ITPK家族基因在各器官中表达量不同,在发育前期的种子、根系和根瘤中表达较高。本研究为花生ITPK基因的后续研究奠定理论基础,为明确ITPK对花生生长中的调控作用提供了依据。1,3,4-triphosphoinositide 5/6-kinase(ITPK)is a conservative multi-function enzyme,which regu⁃lates the metabolism of the inositol phosphate,and is widely existent in plant,animal and nematode.In this study,7 AdITPKs and 7 AiITPKs were obtained from the wild species peanut genome Arachis duranensis and Arachis ipaensis,and the biological characteristics of the ITPK gene family of peanut were analyzed by bioinformatics meth⁃od.The results showed that the chromosome location of AdITPKs and AiITPKs gene was similar between the two speices,both two members on chromosome 03 and 05,one AdITPK on the chromosomes A01,A08 and A10,and one AiITPK on the chromosomes B01,B07,B10,respectively.The exons of the ITPK genes in peanut between 1 to 10,encoded with 220 to 483 amino acids,the evolution relationship analysis showed that the ITPK gene family were divided into 3 subfamilies,based on the conservative domain analysis,the family gene contained 4 to 6 conservative structural motifs.The secondary structure of the two homologous genes was similar,except AdITPK5 and AiITPK5、AdITPK6 and AiITPK6.The tertiary structure of the most peanut ITPKs were similar,while AdITPK1、AdITPK6、AiITPK1 and AiITPK6 were significantly different from the others.The tissue expression pattern of the peanut ITPK is different,and the expression levels of the peanut ITPKs were high in the seed,the root and the root nodule in the early growth period.This study laid a theoretical foundation for the later study of peanut ITPK gene and provided a basis for the regulation of ITPK on peanut growth.

关 键 词：花生 1 3 4-三磷酸肌醇5/6激酶(ITPK) 生物信息学分析 

分 类 号：S565.2[农业科学—作物学]