向日葵NBS-LRR抗病基因家族全基因组分析  

Genome-wide analysis of NBS-LRR-encoding gene in Helianthus annuus

作　　者：路妍 刘洋[1] 宋阳[1] 景岚[1] LU Yan;LIU Yang;SONG Yang;JING Lan(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010019,China)

机构地区：[1]内蒙古农业大学园艺与植保学院,内蒙古呼和浩特010019

出　　处：《中国油料作物学报》2020年第3期441-452,共12页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31760509)。

摘　　要：利用生物信息学方法对向日葵基因组的蛋白质数据库进行搜索,共获得255个富含亮氨酸的重复序列和核苷酸结合位点(nucleotide binding site and leucine rich repeat,NBS-LRR)类抗病基因的蛋白质序列,并根据结构域类型,将其分为TIR和Non-TIR两类。进一步对其中42个同时含有LRR和NBS结构域的LRR-NBS型基因编码蛋白质的理化性质、氨基酸序列及基因在染色体上的物理分布进行分析,结果表明,基因多分布于4号、8号和13号染色体,而且52.4%以基因簇存在;结构域预测结果显示,该类蛋白都具有特征结构域NBS结构域,氨基酸序列同源性为29.26%。对6个NBS类基因经向日葵锈菌诱导后的表达分析表明,6个基因均可被向日葵锈菌诱导表达,说明其可能参与了向日葵响应锈菌胁迫的抗病反应过程。In this study,255 NBS-encoding genes were identified in sunflower genome by bioinformatics methods.The results showed that the candidate proteins could be classified into two groups,based on the gene structural diversity,conserved protein motifs and phylogenetic relationship analysis.We also identified physic-chemical properties,motifs of encoded putative proteins,and physical locations of 42 NBS-LRR genes.We found that most of NBS-LRR genes were located in chromosome 4,8 and 13,52.4%of which were located in gene clusters.Additionally,domain prediction data showed all the proteins had NBS domain.These 42 genes shared 29.26%similarity in amino acid sequence.Expression data revealed all of six NBS genes had specific expression in disease-resistant varieties which inoculated with Puccinia helianthi.The results indicated that these genes might be involved in the sunflower resistant response to rust disease stress.

关 键 词：向日葵 NBS-LRR 抗病基因 向日葵锈病 

分 类 号：S432.2[农业科学—植物病理学]