GA3预处理对野生大豆荫蔽响应的影响  

Effects of GA3 pre-treatment on shade response of Glycine soja

作　　者：蒋亨珂 孙孟园 黎艳 孙歆[1] 尚静 余靓 刘春燕[1] 杨文钰[1] 杜俊波[1] JIANG Heng-ke;SUN Meng-yuan;LI Yan;SUN Xin;SHANG Jing;YU Liang;LIU Chun-yan;YANG Wen-yu;DU Jun-bo(College of Agronomy,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学农学院,四川成都611130

出　　处：《中国油料作物学报》2020年第3期472-479,共8页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31871552,31671445);四川省科技计划资(2018HH0108)。

摘　　要：野生大豆常生长在灌木遮荫环境下,具有耐荫特性,但其适应荫蔽的机理却未被报道。赤霉素(GA3)是调控植物避荫反应的主要激素之一,为探索赤霉素在野生大豆响应荫蔽环境中的作用,本文以赤霉素及其抑制剂多效唑(PAC)预处理野生大豆幼苗,比较白光和荫蔽条件下野生大豆的表型差异,并通过qPCR检测不同处理下光合色素合成基因(GsCAO)、光合电子传递过程调控基因(GsPSAE和GsPSAG)的表达情况。结果表明,GA3预处理会使野生大豆幼苗对荫蔽更加敏感,而荫蔽下经PAC预处理的野生大豆幼苗光合色素含量显著高于对照,与GA3预处理相反。叶绿素荧光参数分析结果显示,PAC预处理能增强野生大豆幼苗的耐荫性,可能与GsCAO、GsPSAE和GsPSAG表达量变化相关。Glycine soja often grow in shrub-shaded environments,but the mechanisms for adapting to shading have not been reported.Gibberellins(GAs)are a group of hormones that regulates shade avoidance.In order to study the effects of GA3 on the shade avoidance of Glycine soja,the phenotype of Glycine soja under white light and shad⁃ing after GA3 and GA synthesis inhibitor(paclobutrazol,PAC)application were analyzed.Expression of genes regu⁃lating photosynthetic pigment biosynthesis,GsCAO,and photosynthetic electron transport,GsPSAE and GsPSAG,were analyzed.The results indicated that photosynthetic pigment content of shading Glycine soja after PAC pretreatment was significantly higher than that of the control,while GA3 pre-treatment were opposite.chlorophyll fluo⁃rescence parameters suggested that PAC pre-treatment could enhance the shade-resistant while GA3 pre-treatment would make Glycine soja more sensitive to shade.Further research demonstrated that the expression of photosynthet⁃ic pigment biosynthesis gene GsCAO,photosynthetic electron transport genes GsPSAE and GsPSAG had an effect on enhanced shade-resistant of Glycine soja.

关 键 词：野生大豆 荫蔽 赤霉素 多效唑 光合色素基因 

分 类 号：S565.1[农业科学—作物学] S311