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作 者:田雪芬 郭文厂 李冬冬 黄秀贞[1] TIAN Xuefen;GUO Wenchang;LI Dongdong;HUANG Xiuzhen(Jining Hospital of TCM,Jining,Shandong,China 272100)
出 处:《中国药业》2020年第11期48-52,共5页China Pharmaceuticals
基 金:山东省济宁市科技助推新旧动能转换计划项目[2017SMNS005]。
摘 要:目的建立二黄益肾汤的质量控制标准。方法原药材经水提、浓缩、纯化;采用薄层色谱鉴别方中大黄、白术2味药材;采用反相高效液相色谱法分别测定丹参素、原儿茶醛、淫羊藿苷的含量。结果薄层色谱法能准确定性鉴定大黄、白术,斑点清晰,阴性对照无干扰;丹参素、原儿茶醛、淫羊藿苷质量浓度分别在6.1~97.6μg/m L(r=1.0000),1.688~27.000μg/m L(r=1.0000),8.063~129.000μg/m L(r=1.0000)范围内与峰面积线性关系良好,平均回收率分别为99.58%,101.44%,99.80%,RSD分别为2.36%,1.30%,1.08%(n=6)。结论该方法简单合理,专属性强,准确可靠,重复性好,定性和定量分析准确,可用于二黄益肾汤的质量控制。Objective To establish the quality control standard for Erhuang Yishen Decoction.Methods Medicinal materials were extracted with water,which were further concentrated and purified.Thin layer chromatography(TLC)was used for the qualitative identification of Rheum officinale and Atractylodes macrocephala.Reversed phase high performance liquid chromatography(RP-HPLC)was used for the quantitative determination of salvianic acid A and protocatechuic aldehyde in Salvia miltiorrhiza,as well as icariin in Epimedium brevicornu.Results TLC could accurately detected the presence of Rheum officinale and Atractylodes macrocephala.The concentration of salvianic acid A,protocatechuic aldehyde and icariin showed good linearity in the ranges of 6.1-97.6μg/m L(r=1.0000),1.688-27.000μg/m L(r=1.0000)and 8.063-129.000μg/m L(r=1.0000),respectively.The average recoveries were 99.58%,101.44%,99.80%,and the RSDs were 2.36%,1.30%,1.08%(n=6),respectively.Conclusion The method is simple and reasonable,strong in specificity,accurate and reliable,good in repeatability,accurate in qualitative and quantitative analysis,and can be used for quality control of Erhuang Yishen Decoction.
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