侵染贵州甘蓝的芜菁花叶病毒CP基因序列分析  

Analysis on CP Gene Sequence of Turnip Mosaic Virus Infecting Cabbage in Guizhou

作　　者：刘学辉[1] 李淳 陈小均[1] 何海永[1] 王莉爽 LIU Xuehui;LI Chun;CHEN Xiaojun;HE Haiyong;WANG Lishuang(Plant Protection Institute,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China)

机构地区：[1]贵州省农业科学院植物保护研究所,贵州贵阳550006

出　　处：《广东农业科学》2020年第4期99-105,共7页Guangdong Agricultural Sciences

基　　金：贵州省科技支撑计划项目(黔科合支撑〔2017〕2576);贵州省基础研究计划项目(黔科合基础〔2017〕1183)。

摘　　要：【目的】明确贵州省甘蓝芜菁花叶病毒(Turnip mosaic virus,TuMV)的发生分布,为科学防控提供指导。【方法】在贵州省安顺市、威宁县和修文县采集甘蓝病毒病样品,采用酶联免疫吸附试验法(enzyme-linked immunosorbent assay,ELISA)和RT-PCR对病样进行TuMV检测,利用相关生物学软件分析TuMV CP基因序列的一致性,并构建系统进化树。【结果】152份甘蓝样品中66份检测出阳性,检出率为43.42%;测序的15个TuMV CP基因核苷酸同源性为88.70%~100%,与已报道TuMV 6个组的分离物构建系统进化树发现,14个分离物属于basal-BR,1个分离物属于world-B组。【结论】TuMV在贵州省甘蓝不同种植区普遍发生,且basal-BR为感染甘蓝的优势毒源。【Objective】The study was to identify the occurrence and distribution of cabbage Turnip mosaic virus(TuMV)in Guizhou Province,and provide guidance for scientific prevention and control.【Method】Samples of cabbage virus disease were collected in Anshun City,Weining County and Xiuwen County of Guizhou Province,and TuMV was detected by enzyme-linked immunosorbent assay(ELISA)and RT-PCR.The consistency of TuMV CP gene sequences was analyzed by relevant biological software,and the phylogenetic tree was constructed.【Result】Among 152 samples of cabbage virus disease,66 samples were tested positive,with a detection rate of 43.42%.The nucleotide homology of 15 TuMV CP genes was 88.70%-100%,and it was found that 14 isolates belonged to basal BR and 1 isolate belonged to world-B group according to the phylogenetic tree construction of isolates in 6 TuMV groups.【Conclusion】TuMV is common in different cabbage planting areas of Guizhou Province and basal-BR is the dominant toxic source for the infection of cabbage.

关 键 词：甘蓝 芜菁花叶病毒 外壳蛋白(CP) 分子变异 

分 类 号：S432.41[农业科学—植物病理学]