弱碱性蒽贝素氨水溶液灌胃对小鼠肝细胞有丝分裂的影响  

Effect of intragastric administration of slightly alkaline embelin and ammonium hydroxide solution on mitosis of mouse hepatocytes

作　　者：何平[1] 李开双 饶伟源 邹佳峻[1] 曾宪彪 HE Ping;LI Kaishuang;RAO Weiyuan;ZOU Jiajun;ZENG Xianbiao(Youjiang Medical University For Nationalities,Baise 533099,China;不详)

机构地区：[1]右江民族医学院检验学院,广西百色533099 [2]广西壮族自治区中医药研究院

出　　处：《山东医药》2020年第15期58-61,共4页Shandong Medical Journal

基　　金：广西自然科学基金项目(2013GXNSFAA019261);广西医疗卫生重点科研课题(重2012057);广西中药质量标准研究重点实验室主任基金(桂中重科201103)。

摘　　要：目的观察弱碱性蒽贝素氨水溶液灌胃对小鼠肝细胞有丝分裂的影响。方法昆明小白鼠50只,随机分为蒽贝素高、中、低剂量组,溶剂组,空白组。蒽贝素高、中、低剂量组分别灌胃浓度0.02、0.01、0.00125 mg/mL的弱碱性蒽贝素氨水溶液20 mL/kg,空白组灌胃蒸馏水20 mL/kg,溶剂组灌胃0.025%氨水溶液(pH 8.2)20 mL/kg,1次/d,连续7 d。末次给药后次日,取肝脏作组织病理学检查,观察分裂相肝细胞核染色体。计数异常分裂肝细胞数和分裂相肝细胞总数,计算异常分裂肝细胞数占分裂相肝细胞总数的百分数。结果小鼠灌胃弱碱性蒽贝素氨水溶液7 d后,组织病理检查见:分裂相肝细胞核质着色特异,核染色体松散,充满胞核,或核染色体散裂成碎片状,不均等分布于胞核两极,或核染色体固缩成细粒状,不均等分布于两个子代肝细胞的非中心位置。蒽贝素高、中剂量组分裂相肝细胞总数、异常分裂肝细胞数、异常分裂肝细胞数占分裂相肝细胞总数的百分比大于溶剂组、空白组(P均<0.05)。结论0.02、0.01 mg/mL碱性蒽贝素氨水溶液对肝细胞有丝分裂有影响,可破坏核质在胞核两极或两子代细胞核中的均等分配,终止肝细胞分裂或使肝细胞死亡。Objective To study the effect of intragastric administration of slightly alkaline embelin and ammonium hydroxide solution on hepatocyte mitosis of mice.Methods We selected 50 Kunming mice.The mice in the high-,medium-,and low-dose embelin groups were given slightly alkaline embelin and ammonium hydroxide solution(20 mL/kg)by gavage at the concentrations of 0.02,0.01,and 0.00125 mg/mL;the mice in the blank group were given 20 mL/kg of distilled water by gavage,and the solvent group with 0.025%ammonia solution(pH 8.2)20 mL/kg,once per day for 7 days.On the next day after the last dose,we took the liver for histopathological examination and observed the nuclear chromosome of the hepatocytes in the divisive phase.We counted the number of abnormal mitotic hetapocytes and the total number of dividing phase hetapocytes.The percentage of abnormal mitotic hetapocytes in the total number of dividing phase hetapocytes was calculated.Results After 7-day treatment of slightly alkaline embelin and ammonium hydroxide solution by means of intragastric administration,histopathology examination showed that unclear material staining was specific in abnormal mitotic hetapocytes,the nuclear chromosomes was in a loose state,or was distributed at both ends poles of cell nucleus in spallation and fragments state,or was distributed in non-central position of the two progeny hetapocytes condensed into fine grains state.The total number of dividing phase hetapocytes,abnormal mitotic hetapocytes,and percentages of abnormal mitotic hetapocytes in the total number of dividing phase hetapocytes in the high-dose and medium-dose embelin groups were greater than those in the solvent group and blank group.Conclusion The slightly alkaline embelin and ammonium hydroxide solution(0.02 and 0.01 mg/mL)has an effect on mitosis of liver cells,which can destroy the equal distribution of nucleus and cytoplasm in the nucleus of two poles or two progeny nucleus,stop liver cell division or cause liver cell death.

关 键 词：蒽贝素 肝细胞 分裂相肝细胞 异常分裂肝细胞 核染色体 

分 类 号：R992[医药卫生—毒理学]