星点设计效应面法优化可溶性糖的提取工艺  

Optimization of extraction process of water soluble carbohydrateby central composite design-response surface methodology

作　　者：丁雪梅[1] 张晓君[2] 刘月[1] 刘小曼[1] 包国章 孙博兴[1] DING Xue-mei;ZHANG Xiao-jun;LIU Yue;LIU Xiao-man;BAO Guo-zhang;SUN Bo-xing(College of Animal Science,Jilin University,Changchun 130062,China;Center of Education Technology,Jilin University,Changchun 130012,China;College of New Energy and Environment,Jilin University,Changchun 130012,China)

机构地区：[1]吉林大学动物科学学院,吉林长春130062 [2]吉林大学教育技术中心,吉林长春130012 [3]吉林大学新能源与环境学院,吉林长春130012

出　　处：《东北师大学报（自然科学版）》2020年第2期124-129,共6页Journal of Northeast Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(31772669);中国高等教育学会理科教育专业委员会教育研究课题(01006);吉林省高教学会高教科研课题(JGJX2017D6);吉林省教育科学“十三五”规划课题(GH180002);吉林大学教学改革项目(2019XYB051,2019XZD078).

摘　　要：采用星点设计效应面法考察了乙醇体积分数(0,17%,40%,63%,80%)、提取温度(60℃,68.5℃,80℃,91.5℃,100℃)和提取时间(15,24.5,37.5,50.5,60 min)对可溶性糖含量的影响,研究了蒽酮比色法测定可溶性糖含量的最佳提取方法.利用Design-Expert.8.0.6软件对实验数据进行二次多项式模型拟合.结果表明:乙醇体积分数和提取时间对可溶性糖含量有显著影响(P<0.05),提取温度无影响(P>0.05),影响次序为:乙醇体积分数>提取时间>提取温度.可溶性糖的最佳提取工艺为:乙醇体积分数54%,提取温度100℃,提取时间60 min.Effect of ethanol volume fracrion(0,17%,40%,63%,80%),extraction temperature(60℃,68.5℃,80℃,91.5℃,100℃)and extraction time(15,24.5,37.5,50.5,60 min)on water soluble carbohydrate content by central composite design-response surface methodology was studied to explore the best extraction method of water soluble carbohydrate by anthrone colorimetry.Design-Expert 8.0.6 statistic analysis software was used to fit a second-order polynomial equation for experimental data.The result showed ethanol volume fracrion and extraction time significantly affected water soluble carbohydrate content(P<0.05),but extraction temperature had not(P>0.05),and the factor that affected soluble carbohydrate content was in the order of ethanol volume fracrion>extraction time>extraction temperature.The optimal extraction process of water soluble carbohydrate was as follows:ethanol concentration was 54%,extraction temperature was 100℃,and extraction time was 60 min.

关 键 词：星点设计效应面法 可溶性糖 提取工艺 蒽酮比色法 模型拟合 

分 类 号：S56[农业科学—作物学]