甘蓝型油菜GPDH基因家族的全基因组鉴定  

Genome-wide Identification of GPDH Gene Family in Brassica napus

作　　者：张超 付三雄[2] 唐容 周小婴[2] 黄莎 王璐璐[1] 杨克相 戚存扣[2] ZHANG Chao;FU Sanxiong;TANG Rong;ZHOU Xiaoying;HUANG Sha;WANG Lulu;YANG Kexiang;QI Cunkou(Guizhou Oil Crops Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China;Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)

机构地区：[1]贵州省农业科学院贵州省油料研究所,贵阳550006 [2]江苏省农科院经济作物研究所,南京210014

出　　处：《西北农业学报》2020年第3期363-373,共11页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：贵州省科学技术基金(黔科合LH字[2014]7685号);贵州省科技计划(黔科合NY[2015]3019-1号);贵州省油菜现代农业产业技术体系资助项目(GZCYTX2018-0801)。

摘　　要：在甘蓝型油菜基因组中鉴定出18个BnGPDH基因,参考拟南芥AtGPDH基因家族分类信息将它们分为BnGPDHp、BnGPDHm和BnGPDHc 3个亚家族。基因结构、保守模块分析发现亚家族内的基因成员具有较高的保守性;染色体位置定位显示BnGPDH分布在10条染色体上;qRT-PCR分析发现BnGPDHc家族成员在所检测的组织部位中具有不同的表达模式。此外,在花后21 d(day after flower,DAF)和30DAF的种子中BnGPDHc1-2、BnGPDHc2-1、BnGPDHc2-2的相对表达量在高油材料中要显著高于低油材料,表明这些基因可能与油菜种子中三脂酰甘油的形成相关。Glycerol-3-phosphate dehydrogenase(GPDH)is one of the rate-limiting enzymes that controlling the synthesis of glycerol-3-phosphate(G3P)in plants,and plays an important role in triacylglycerol(TAG)accumulation.In this study,18 BnGPDH genes were identified from genome database of B.napus,which were classified into three subfamilies,including BnGPDHp,BnGPDHm and BnGPDHc,by referring to Arabidopsis gene family classification information.Gene structure and conservative module analysis indicated that BnGPDH genes were highly conservative in subfamily.The chromosome mapping analysis showed that BnGPDH genes were located in 10 chromosomes.The qRT-PCR analysis revealed that BnGPDHc genes had different expression patterns in the tested tissues and organs.Moreover,BnGPDHc1-2,BnGPDHc2-1 and BnGPDHc2-2 were preferentially expressed in high-oil accessions rather than low-oil accessions in the seeds of 21 DAF(day after flowering)and 30 DAF,and it was possibly related to TAG formation of seeds.This research lays the foundation for further study about function of GPDH genes.

关 键 词：甘蓝型油菜 三磷酸甘油脱氢酶 全基因组鉴定 实时荧光定量PCR 

分 类 号：S565.4[农业科学—作物学]