以居住地域为基础的广州地区结核病传播链分子流行病学初探  

Preliminary explore on molecular epidemiology of tuberculosis transmission chains in Guangzhou based on residence

作　　者：竺澎波[1] 王楠[1] 孟繁荣[1] 杨瑜[1] 雷杰[1] 牛群[1] 谢贝[1] 吴玲[1] 李华 刘志辉[1] 刘文 Zhu Pengbo;Wang Nan;Meng Fanrong;Yang Yu;Lei Jie;Niu Qun;Xie Bei;Wu Ling;Li Hua;Liu Zhihui;Liu Wen(Guangzhou Chest Hospital,Guangzhou 510095;Huadu District People's Hospital,Guangzhou 510800,China)

机构地区：[1]广州市胸科医院,510095 [2]广州市花都区人民医院,510800

出　　处：《国际医药卫生导报》2020年第12期1649-1654,共6页International Medicine and Health Guidance News

基　　金：“艾滋病和病毒性肝炎等重大传染病防治”国家科技重大专项(2018ZX10715004);广东省转化医学创新平台建设项目(省卫函[2018]1254号);广州市高水平临床重点专科和培育专科建设项目(穗卫函[2019]155号);广州市卫生健康科技项目(20201A011042)。

摘　　要：目的探讨MIRU-VNTR基因分型技术用于分析广州地区结核病传播关系的可行性。方法以结核患者居住地所属乡镇村组或社区街道相同为前提,假定居住所属地相同结核患者间存在传播关系,拟定广州地区结核病传播链。培养结核分枝杆菌临床分离株并提取DNA,通过PCR扩增进行29位点MIRU-VNTR基因分型分析,并计算HGDI分辨力指数。以基因型成簇与否评价传播链内结核患者间传播关系的有无。结果拟定了178条广州地区结核病传播链,复苏得到456株结核分枝杆菌临床分离株,提取获得其基因组DNA并扩增了29个VNTR位点,其组合HGDI分辨力指数为0.986。基因成簇性分析显示,有2条传播链内患者结核分枝杆菌临床分离株基因成簇,成簇率为1.10%。基因成簇性的2条传播链患者分别居住于同街道,发病时间间隔在2年以内,分别为9和14个月。结论29位点MIRU-VNTR基因分型技术可用于广州地区结核分枝杆菌基因型分析,能否用于结核病传播关系需要进一步深入研究。Objective To explore the feasibility of MIRU-VNTR genotyping technology for analyzing the transmission of tuberculosis(TB)in Guangzhou.Methods Based on the premise that TB patients lived in the same area,such as same villages and towns or the same community streets,might had transmission relations,the TB transmission chains in Guangzhou were drawn up.The clinical isolates of Mycobacterium tuberculosis were cultured,and the DNA was extracted.The 29-locus MIRU-VNTR genotyping analysis was performed by PCR amplification,and the HGDI resolution index was calculated.Genotype clustering was used to evaluate the presence or absence of transmission relationships among tuberculosis patients in the transmission chains.Results 178 TB transmission chains in Guangzhou were drawn up.456 clinical isolates of Mycobacterium tuberculosis from resuscitated patients were amplified to obtain 29 VNTR sites,and the combined HGDI resolution index was 0.986.Gene clustering analysis showed that there were 2 transmission chains in which the genes of clinical isolates of Mycobacterium tuberculosis were clustered,with a clustering rate of 1.10%.The patients in the two transmission chains of gene clustering lived in the same street,and their onset time intervals was within 2 years,which were 9 months and 14 months,respectively.Conclusion 29-locus MIRU-VNTR genotyping technique can be used to analyze the genotype of Mycobacterium tuberculosis in Guangzhou,and whether it can be applied to the relationship between tuberculosis transmission needs further in-depth study.

关 键 词：结核 结核分枝杆菌 传播链 MIRU-VNTR 基因成簇性 

分 类 号：R52[医药卫生—内科学]