川牛膝不同产地药材质量综合评价研究  

Study on quality evaluation of Cyathula officinalis Kuan

作　　者：丁刚[1,2,3,4] 李隆云 宋旭红[1,2,3,4] 付绍智 DING Gang;LI Long-yun;SONG Xu-hong;FU Shao-zhi(Chongqing Academy of Chinese Materia Medica;Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica;Chongqing key Laboratory of Chinese Medicine Resources;Chongqing Sub-center of National Resources Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Chongqing 400065,China;Chongqing Three Gorges Medical College,Chongqing 404120,China)

机构地区：[1]重庆市中药研究院 [2]重庆市中药良种选育与评价工程技术研究中心 [3]重庆市中药资源学重点实验室 [4]中国中医科学院中药资源中心重庆分中心,重庆400065 [5]重庆三峡医药高等专科学校,重庆404120

出　　处：《天然产物研究与开发》2020年第5期851-859,共9页Natural Product Research and Development

基　　金：国家中药材产业技术体系(CARS-21);重庆市中药材产业技术体系(2019-[5]号);重庆市技术创新与应用发展专项(cstc2019jscx-gksbX0009);奉节川牛膝规范化种植关键技术研究与示范推广(cstc2016shmszx1128)。

摘　　要：为了建立更全面的川牛膝药材质量评价方法,按照2015版《中华人民共和国药典》方法测定水分、总灰分、水冷浸物和乙醇冷浸物;新建HPLC法测定川牛膝中杯苋甾酮、前杯苋甾酮和齐墩果酸3种成分的含量,并通过SPSS 20.0软件进行聚类分析和主成分分析。本研究较全面地评价了全国54个产地川牛膝药材的质量,54批川牛膝样品的水分、总灰分、浸出物、含量均符合药典标准,为川牛膝药材的进一步开发提供了依据。In order to establish a more comprehensive quality evaluation method of Cyathula officinalis Kuan.The water content,total ash,water-cooled extract and ethanol cold extract were determined according to the Pharmacopoeia of the people's Republic of China(2015);A new HPLC method was established for the determination of three components of cyasterone precyasterone and oleanolic acid in C.officinalis.custer analysis and principal component analysis were carried out by SPSS 20.0 software.This study comprehensively evaluated the quality of C.officinalis from 54 places in China,the water content,total ash,extract and content of 54 batches of C.officinalis samples all meet the pharmacopoeia standard,provided a basis for the further development of C.officinalis.

关 键 词：川牛膝 质量评价 聚类分析 主成分分析 

分 类 号：R932[医药卫生—生药学]