IL-2/GM-CSF体外处理的外周血单个核细胞对再生障碍性贫血小鼠T淋巴细胞亚群及细胞因子的影响  

Effects of peripheral blood mononuclear cells treated with IL-2 and GM-CSF in vitro on T lymphocyte subsets and cytokines in aplastic anemia mice

作　　者：詹昱[1] 曲佳[1] 冯可欣[1] 杨郁青[1] 谭明珠[1] 谭雪芳[1] 杨德懋[1] Yu Zhan;Jia Qu;Ke-xin Feng;Yu-qing Yang;Ming-zhu Tan;Xue-fang Tan;De-mao Yang(Department of Hematology,Guangzhou Twelfth People’s Hospital,Guangzhou,Guangdong 510620,China)

机构地区：[1]广州市第十二人民医院血液科,广东广州510620

出　　处：《中国现代医学杂志》2020年第12期1-6,共6页China Journal of Modern Medicine

基　　金：广东省医学科学技术研究基金(No:A2017499)。

摘　　要：目的探讨白细胞介素-2(IL-2)/粒细胞-巨噬细胞集落刺激因子(GM-CSF)体外处理的外周血单个核细胞输注对再生障碍性贫血(以下简称再障)小鼠T淋巴细胞亚群和细胞因子的影响及其促造血作用。方法选取清洁级雌性BALB/c小鼠并复制免疫介导再障小鼠模型,设立对照组、模型组及干预组。干预组取正常同源小鼠外周血单个核细胞,在IL-2、GM-CSF等作用下培养48 h后进行尾静脉输注(模型复制第1、2、4及6天),模型组输注等体积生理盐水。全部小鼠分别于模型复制第7、14、21及28天断尾采血检测外周血白细胞、血红蛋白和血小板计数;于模型复制第28天或濒死时处死小鼠,计数骨髓有核细胞(粒系、红系、淋巴细胞比例及巨核细胞数),检测外周血CD3+CD4+(T辅助细胞、Th细胞)、CD3+CD8+(T抑制细胞、Ts细胞)及CD4+CD25+CD127-(调节性T细胞、T-regs)细胞比例,检测血清肿瘤坏死因子-α(TNF-α)、γ干扰素(IFN-γ)水平。结果模型组和干预组外周血WBC较对照组低(P<0.05),干预组在第21和28天较模型组高(P<0.05)。模型组和干预组外周血PLT较对照组低(P<0.05),干预组在第21和28天较模型组高(P<0.05)。模型组和干预组外周血Hb较对照组低(P<0.05),干预组在第28天较模型组高(P<0.05)。模型组和干预组粒系比例、红系比例及巨核细胞数较对照组低,淋巴细胞比例较对照组高(P<0.05),干预组骨髓粒系比例、红系比例及巨核细胞数较模型组高,淋巴细胞比例较模型组低(P<0.05)。模型组Th细胞比例、T-regs细胞比例较对照组低(P<0.05),Ts细胞比例较对照组高(P<0.05)。干预组Th细胞比例、T-regs细胞比例较模型组高,Ts细胞比例较模型组低(P<0.05)。模型组外周血TNF-α、IFN-γ水平较对照组高(P<0.05),干预组较模型组低(P<0.05)。结论IL-2/GM-CSF体外处理的外周血单个核细胞输注有助于调节再障小鼠T淋巴细胞亚群紊乱,下调造血负调控细胞因�Objective To explore the effects of peripheral blood mononuclear cells treated with IL-2 and GM-CSF in vitro on T lymphocyte subsets and cytokines in aplastic anemia mice.Methods Models of immune-mediated aplastic anemia were established according to literature methods,and a control group,a model group and an intervention group were established.Peripheral blood mononuclear cells were isolated from normal homologous mice and cultured for 48 hours under the effects of IL-2 and GM-CSF and administered intravenously to mice in the intervention group(on the 1st d,2nd d,4th d and 6th d).The model group was given equal volume of normal saline.The counts of WBC,PLT,and Hb level in peripheral blood were tested in all mice on the 7th d,14th d,21st d,and 28th d.All mice were sacrificed on the 28th d or the brink of death.The numbers of bone marrow nucleated cells(granulocytes,erythrocytes,lymphocytes,megakaryocytes)were counted.The proportions of CD3+CD4+(T helper cells,Th cells),CD3+CD8+(T suppressor cells,Ts cells),CD4+CD25+CD127−(regulatory T cells,T-regs)cells in peripheral blood were measured,and the levels of TNF-αand IFN-γin serum were measured.Results The WBC of the model group and the intervention group was lower than that of the control group(P<0.05),and the WBC of the intervention group was higher than that of the model group on the 21st and 28th d(P<0.05).PLT in the model group and the intervention group was lower than that in the control group(P<0.05),and higher in the intervention group on the 21st and 28th day(P<0.05).The Hb of model group and intervention group was lower than that of control group(P<0.05),and the Hb of intervention group was higher than that of model group on the 28th day(P<0.05).The proportion of granulocytes,erythrocytes and megakaryocytes in the model group and the intervention group were lower than those in the control group,and the proportion of lymphocytes in the intervention group was higher than that in the control group(P<0.05).The proportion of Th cells and T-regs cells in the

关 键 词：贫血 再生障碍性 白细胞介素2 集落刺激因子 单核细胞 

分 类 号：R556.5[医药卫生—血液循环系统疾病]