4种昆虫病原线虫对草地贪夜蛾的致死作用  

Virulence of four entomopathogenic nematodes on fall armyworm,Spodoptera frugiperda(J.E.Smith)(Lepidoptera:Noctuidae)

作　　者：梁铭荣 李子园 戴钎萱 陆永跃[1] 陈科伟[1] 王磊[1] LIANG Mingrong;LI Ziyuan;DAI Qianxuan;LU Yongyue;CHEN Kewei;WANG Lei(College of Agriculture,South China Agricultural University,Guangzhou,Guangdong 510642,China;College of Plant Protection,Hunan Agricultural University,Changsha,Hunan 410128,China)

机构地区：[1]华南农业大学农学院,广东广州510642 [2]湖南农业大学植物保护学院,湖南长沙410128

出　　处：《生物安全学报》2020年第2期82-89,共8页Journal of biosafety

基　　金：广东省重点领域研发计划(2020B020223004);广东省外来入侵物种风险评估和监测预警共性关键技术研发创新团队建设项目(2019KJ134);省级农业科技创新及推广项目(2018LM2161)。

摘　　要：【目的】探讨昆虫病原线虫对草地贪夜蛾幼虫的侵染效果。【方法】在室内条件下采用生测试验法,测定了小卷蛾斯氏线虫AⅡ、长尾斯氏线虫X-7、夜蛾斯氏线虫SN和嗜菌异小杆线虫H06等4种昆虫病原线虫对草地贪夜蛾2龄和5龄幼虫的致死作用。【结果】小卷蛾斯氏线虫AⅡ与草地贪夜蛾数量比为50∶1时,36 h后草地贪夜蛾2龄和5龄幼虫的死亡率分别为92%和100%。长尾斯氏线虫X-7与草地贪夜蛾数量比为50∶1时,36 h后草地贪夜蛾2龄幼虫的死亡率为80%;在数量比30∶1时,36 h后草地贪夜蛾5龄幼虫死亡率为100%。夜蛾斯氏线虫SN与草地贪夜蛾数量比为400∶1时,36 h后草地贪夜蛾2龄和5龄幼虫的死亡率分别为88%和85%。嗜菌异小杆线虫H06与草地贪夜蛾在400∶1时,36 h后草地贪夜蛾2龄和5龄幼虫的死亡率分别为32%和67.5%。【结论】小卷蛾斯氏线虫AⅡ具有较好的草地贪夜蛾生物防治潜质,其次是长尾斯氏线虫X-7。【Aim】This study aimed to examine the infection effect of entomopathogenic nematode on the 2 nd and 5 th instar larvae of Spodoptera frugiperda(J.E.Smith).【Method】We used a bioassay to test the virulence of four entomopathogenic nematodes:Steinernema carpocapsae AⅡ,S.longicaudum X-7,S.feltiae SN and Heterorhabditis bacteriophora H06,under laboratory conditions.【Result】After exposing one S.frugiperda larva to 50 S.carpocapsae AⅡfor 36 hours,the mortality rates were 92%for 2 nd and 100%for 5 th instar S.frugiperda larvae.When exposed to 50 S.longicaudum X-7,the mortality of 2 nd instar S.frugiperda larvae was 80%and 100%for 5 th instar S.frugiperda larvae in the presence of only 30 S.longicaudum X-7.After exposing one S.frugiperda larva face to 400 S.feltiae SN for 36 hours,the mortality rates of 2 nd and 5 th instar S.frugiperda larvae were 88%and 85%and 32%and 67.5%in the presence of 400 H.bacteriophora H06.【Conclusion】The results showed that S.carpocapsae AⅡand S.longicaudum X-7 had great potential on biological control of 2 nd and 5 th instar S.frugiperda larvae.

关 键 词：昆虫病原线虫 草地贪夜蛾 致死作用 生物防治 

分 类 号：S476.15[农业科学—农业昆虫与害虫防治] S433.4[农业科学—植物保护]