Anammox bacterial abundance and biodiversity in greenhouse vegetable soil are influenced by high nitrate content  

作　　者：Hongyi QIN Huan DENG Cheng HAN Wenhui ZHONG 

机构地区：[1]School of Geography Science,Nanjing Normal University,Nanjing 210023,China [2]School of Environment,Nanjing Normal University,Nanjing 210023,China [3]Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control,Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application,Nanjing 210023,China

出　　处：《Pedosphere》2020年第3期343-351,共9页土壤圈（英文版）

基　　金：This study was funded by the National Natural Science Foundation of China(Nos.41401293 and 41771286),the National Science and Technology Pillar Program of China(No.2013BAD11B01),and the National Key Research and Development Program of China(No.2016YFD0200302).The authors thank Professor Peter Bottomley from Oregon State University,USA for language editing of the manuscript.

摘　　要：The anaerobic ammonium oxidizing(anammox) process has been found to play an important role in terrestrial ecosystems in recent years. However,the diversity and abundance of anammox bacteria in nitrogen(N)-rich agricultural soils under high fertilizer greenhouse conditions are still unclear. Two greenhouse fields with different N fertilizer input levels were chosen, and their soil profiles were studied with molecular technologies, including quantitative polymerase chain reaction assay, a clone library, and phylogenetic analysis based on hzsB(encoding anammox hydrazine synthase β-subunit) gene. Molecular analyses suggested that anammox bacteria were at their highest density at 10–20 cm soil depth, and that the anammox bacterial abundance was significantly lower at high N than at low N. Candidatus Brocadia was the sole anammox bacterial genus throughout the soil depth profiles. The highest diversity of anammox bacteria was found at 30–40 cm soil depth, and different phylotypic clusters of Candidatus Brocadia were associated with specific soil environmental factors, such as nitrates, soil depth, and total N. Correlation analyses and redundancy analyses confirmed that high nitrate content associated with high N fertilizer input had a significant negative influence on the abundance and biodiversity of anammox bacteria. These results imply that excessive use of N fertilizer would affect arid land soil N loss to the atmosphere by the anammox pathway.

关 键 词：anammox pathway community structure fertilizer input level greenhouse soil hydrazine gene nitrogen fertilizer nitrogen pollution soil degradation 

分 类 号：S626[农业科学—园艺学] S154.3