山东省3个野生大豆居群生育期基因遗传多样性分析  

Genetic Diversity Analysis of Wild Soybean Resources from Three Populations of Shandong Province by Using the Functional Markers Targeting the Growth Period-Related Genes

作　　者：刘月 齐广勋[3] 桑永生 王英男[2] 李玉秋[2] 王玉民[2] 刘晓冬[2] 赵洪锟[3] 袁翠平[2] 董英山[2] LIU Yue;QI Guang-xun;SANG Yong-sheng;WANG Ying-nan;LI Yu-qiu;WANG Yu-min;LIU Xiao-dong;ZHAO Hong-kun;YUAN Cui-ping;DONG Ying-shan(College of Agriculture,Jilin Agricultural University,Changchun 130118;Soybean Research Institute,Jilin Academy of Agricultural Sciences,Changchun 130124;Crop Germplasm Resources Institute,Jilin Academy of Agricultural Sciences,Gongzhuling 136100)

机构地区：[1]吉林农业大学农学院,长春130118 [2]吉林省农业科学院大豆研究所,长春130124 [3]吉林省农业科学院作物资源研究所,公主岭136100

出　　处：《植物遗传资源学报》2020年第3期560-567,共8页Journal of Plant Genetic Resources

基　　金：吉林省农业科技创新工程(CXGC2018JC003,CXGC2017ZY006);吉林省农业科技创新工程人才基金项目(C92070402)。

摘　　要：以来自山东省3个居群的137份野生大豆种质资源为试验材料,利用等位基因特异性DNA标记进行生育期E1~E4基因型鉴定及遗传多样性分析。结果表明:在山东省野生大豆生育期基因E1~E4中,E1、E4位点上只检测出1个等位变异,皆为显性基因型E1、E4;E2、E3位点上变异较为丰富,分别以E2-in、E3-Ha为主;共发现5种基因型组合,其中E1E2-dlE3-HaE4基因型为优势组合。3个居群遗传变异不尽相同,临沂居群E2、E3位点以E2-in、E3-Mi为主,共有4种基因型组合,基因型组合以E1E2-inE3-MiE4为主。蓬莱和荣成居群,E2、E3位点以E2-dl、E3-Ha为主,共有3种基因型组合,以E1E2-dlE3-HaE4为主。在遗传多样性方面,山东荣成和蓬莱的亲缘关系较近,与临沂亲缘关系较远。遗传多样性最高的为临沂居群,其次是蓬莱居群,荣成居群。本研究结果可为山东省野生大豆种质资源利用提供理论依据,具有重要的应用价值。The genetic diversity analysis of 137 wild soybean accessions from three populations of Shandong province were analyzed by allele-special DNA markers.One dominant allele was detected at E1 and E4 loci,respectively,while the haplotypes E2-in and E3-Ha were found to be predominant at E2 and E3 loci,respectively.Out of five genotypic combinations,the genotype carrying E1E2-dlE3-HaE4 was predominant.By further analyzing the diversity among the three populations,four genotypic combinations were detected in Linyi population.The genotypes carrying E1E2-inE3-MiE4 were often detected,while E2-in and E3-Mi were major alleles at E2 and E3 loci,respectively.Three genotypic combinations were observed in both Penglai and Rongcheng populations,while E1E2-dlE3-HaE4 was often detected.The alleles E2-dl and E3-Ha were superiority allele on E2 and E3 loci,respectively.A similarity on genetic diversity was found between Rongcheng and Penglai populations,while both were differing with Linyi population that represented a highest genetic diversity.Taken together,the results gained from this study might provide insights for the utilization of wild soybean germplasm resources in Shandong Province.

关 键 词：野生大豆 E基因 遗传多样性 相关性 

分 类 号：S565.1[农业科学—作物学]