新乡市人类免疫缺陷病毒抗体有反应者胶体硒法与蛋白质免疫印迹试验结果分析  

Analysis on colloidal selenium method and Western Blot results of human immunodeficiency virus antibody responders in Xinxiang City

作　　者：徐璐 吕晓静[2] 程栋[1] 于贺军[1] Xu Lu;Lyu Xiaojing;Cheng Dong;Yu Hejun(Department of Accuired Immune Deficiency Syndrome Prevention and Control,Xinxiang Center for Disease Control and Prevention,Xinxiang 453000,Henan,China;Department of Public Health,Xinxiang Center for Disease Control and Prevention,Xinxiang 453000,Henan,China)

机构地区：[1]新乡市疾病预防控制中心性病艾滋病防治科,河南新乡453000 [2]新乡市疾病预防控制中心公共卫生科,河南新乡453000

出　　处：《实用检验医师杂志》2020年第2期114-115,共2页Chinese Journal of Clinical Pathologist

基　　金：河南省医学科技攻关计划项目(201602311)。

摘　　要：目的探讨胶体硒法人类免疫缺陷病毒(HIV)抗体筛查与蛋白质免疫印迹试验(Western Blot)结果的相关性。方法采用胶体硒法对2018-2019年新乡市获得性免疫缺陷综合征(AIDS)筛查实验室送检的610例HIV感染待确定样本进行胶体硒法复检,并对所有样本进行确证实验(Western Blot),采用SPSS 21.0软件进行统计学分析,收集受检者的基本信息,比较两种方法的阳性率。结果 610例患者样本中经胶体硒法检测有反应样本共427例;经Western Blot检测阳性样本377例,阴性样本155例,不确定样本78例。采用胶体硒法所得有反应样本比例明显高于Western Blot法〔70.0%(427/610)比61.8%(377/610)〕,差异有统计学意义(P<0.05)。结论使用胶体硒法进行HIV抗体筛查阳性率高于Western Blot,胶体硒作为一种快速检测试剂,其检测结果可以及早对母婴阻断、职业暴露处置等特殊情况是否进行抗病毒药物干预提供一定参考。Objective To explore the correlation between colloidal selenium method for human immunodeficiency virus(HIV)antibody screening and Western Blot results.Methods The 610 to-be-confirmed samples of HIV infection from different accuired immune deficiency syndrome(AIDS)screening laboratories in Xinxiang City during 2018 to 2019 were retested by colloidal selenium method,and all samples were confirmed by confirmatory experiment(Western Blot).SPSS 21.0 software was used for statistical analysis.The basic information was collected,and the positive rates by two methods were compared.Results Among 610 samples,427 cases were reactive by colloidal selenium method;377 cases were positive by Western Blot,155 cases were negative,78 cases were uncertain.The reactive rate by colloidal selenium method was significantly higher than that by Western Blot[70.0%(427/610)vs.61.8%(377/610)],with significant difference(P<0.05).Conclusions The positive rate of HIV antibody screening by colloidal selenium method was higher than that by Western Blot.Colloidal selenium,as a rapid detection reagent,can be used as a reference for antiviral drugs intervention in special cases such as mother infant block and occupational exposure.

关 键 词：人类免疫缺陷病毒 胶体硒 蛋白质免疫印迹试验 

分 类 号：R512.91[医药卫生—内科学] R446.6[医药卫生—临床医学]