osa-miR168a-5p靶向调节人源ADD1及E2F2基因表达的研究  

Study on osa-miR168a-5p Targeted Regulation of Human ADD1 and E2F2 Gene Expression

作　　者：陈真真 蔡军 CHEN Zhen-zhen;CAI Jun(Fuwai Hospital of Chinese Academy of Medical Sciences/National Cardiovascular Disease Center/State Key Laboratory of Cardiovascular Diseases/Hypertension Center,Beijing 100037,China)

机构地区：[1]中国医学科学院阜外医院/国家心血管病中心/心血管疾病国家重点实验室/高血压中心,北京100037

出　　处：《医学信息》2020年第11期64-66,共3页Journal of Medical Information

基　　金：国家自然科学基金(编号:81800367)。

摘　　要：目的探究植物osa-miR168a-5p靶向调节人源相关基因表达的影响。方法采用TargetScan预测osa-miR168a-5p的靶基因,并筛选相关靶基因进行后续验证。构建靶基因mRNA 3'-UTR荧光素报告基因及相应的突变体,采用双荧光素酶报告基因活性分析系统验证osa-miR168a-5p与靶基因的关系。结果利用Targetscan预测的osa-miR168a-5p靶基因结果显示,ADD1与E2F2mRNA3'-UTR区域均存在osa-miR168a-5p结合位点。双荧光素酶报告基因实验结果显示,使用osa-miR168a-5p mimics内源性增加osa-miR168a-5p能够抑制ADD1及E2F2报告基因的表达,将ADD1及E2F2 mRNA 3'UTR区与osa-miR-168a-5p的结合位点突变后,内源性osa-miR-168a-5p增加所引起的报告基因表达下调的作用消失。结论osa-miR168a-5p能够直接调节人源ADD1及E2F2基因表达,因此食物中的osa-miR168a进入人体很可能直接靶向人源ADD1及E2F2基因发挥相应的生物学效应。Objective To explore the effect of plant osa-miR168a-5p on targeted regulation of human-related gene expression.Methods TargetScan was used to predict osa-miR168a-5p target genes,and related target genes were screened for subsequent verification.The target gene mRNA 3'-UTR luciferin reporter gene and corresponding mutants were constructed,and the dual luciferase reporter gene activity analysis system was used to verify the relationship between osa-miR168a-5p and the target gene.Results The results of osa-miR168a-5p target genes predicted by Targetscan showed that both ADD1 and E2F2mRNA 3'-UTR regions had osa-miR168a-5p binding sites.The results of the dual luciferase reporter gene experiment showed that the endogenous increase of osa-miR168a-5p mimics using osa-miR168a-5p can inhibit the expression of ADD1 and E2F2 reporter genes.After mutating the binding site of ADD1 and E2F2 mRNA 3'UTR region to osa-miR-168a-5p,the effect of the endogenous increase of osa-miR-168a-5p on reporter gene expression disappeared.Conclusion osa-miR168a-5p can directly regulate the expression of human ADD1 and E2F2 genes.Therefore,the entry of osa-miR168a in food into the human body is likely to directly target the human ADD1 and E2F2 genes to exert the corresponding biological effects.

关 键 词：osa-miR168a ADD1 E2F2 靶基因 

分 类 号：R3[医药卫生—基础医学]