八角茴香渗漉法的工艺优化及莽草酸的薄层色谱鉴别  

Process Optimization of Percolation Method and TLC Identification of Shikimic Acid in Star Anise

作　　者：周邦华 龙杰凤 王小羽 汤承浩 谭荣[1] 李本鹏[1] 王翔[1,2] ZHOU Bang⁃hua;LONG Jie⁃feng;XIE Shen⁃qin;WANG Xiao⁃yu;TANG Cheng⁃hao;TAN⁃Rong;Li Ben⁃peng;WANG⁃Xiang(Kaili University,Kaili,Guizhou,556011,China;Qiandongnan Engineering and Technology Research Center for Comprehensive Utilization of Ethnic Medicine,Kaili University,Kaili,Guizhou,556011,China;Shenyang Pharmaceutical University,Shenyang,Liaoning,117000,China)

机构地区：[1]凯里学院,贵州凯里556011 [2]凯里学院黔东南民族药综合利用工程技术研究中心,贵州凯里556011 [3]沈阳药科大学,辽宁沈阳117000

出　　处：《凯里学院学报》2020年第3期45-52,共8页Journal of Kaili University

基　　金：黔东南州科技计划项目(黔科合J字(2019)132号);贵州省教育厅青年科技人才成长项目(黔教合KY字[2019]193号);贵州省基础研究计划(科学技术基金)项目(黔科合基础[2020]1Y387);大学生创新创业训练计划项目(LX201943);贵州省全国第四次中药资源丹寨县普查专项(HX201805);黔东南州国家火炬苗侗医药特色产业基地项目(黔东南科合基地J字[2018]007号)。

摘　　要：为了优化八角茴香中莽草酸渗漉法提取工艺参数,建立提取物TLC法.通过单因素试验和L9(34)正交试验设计-综合评分法,以莽草酸和干膏率为评价指标,采用渗漉法、UV法考察乙醇浓度、料液比、提取时间、药材粒度、渗漉速度与渗漉次数对工艺条件的影响,并对莽草酸进行TLC鉴别.结果发现取八角茴香粉末(80目),加料液比1:15 g/mL的60%乙醇渗漉提取12 h,流速1 mL/min,渗漉1次,测得3批样品中莽草酸的平均含量为9.40%;该样品的TLC鉴别斑点清晰,分离度较好,与文献一致.因此认为本实验优化的工艺条件操作简捷,稳定可行,具有实际应用价值.To optimize the extraction parameters of shikimic acid via percolation method while establishing TLC method of the extraction from Star Anise.The single factor experiment,L9(34)orthogonal test design and comprehensive score method as well as the method of diacolation and UV were used to estimate the influence of diacolation process conditions under ethanol concentration,solid-liquid ratio,extraction time,medicinal material granularity,diacolation speed and times while regarding the rate of shikimic acid and dried extract as the evaluation index.Meanwhile,shikimic acid of the extracts was identified by TLC.Results show that Anise powder(80 mesh)was percolated for 12 hours with solid-toliquid ratio 1:15(g/mL)with 60%ethanol at a flow rate of 1 mL/min for one time.The average content of shikimic acid in 3 batches of samples was 9.40%.The clear TLC identification of the sample and good separated degree were in accordance with the literature.Therefore,it is considered that the optimized technological conditions in this experiment are simple,stable and practical.

关 键 词：八角茴香 渗漉法提取工艺 正交试验TLC法 莽草酸 

分 类 号：O657.7[理学—分析化学] TQ28[理学—化学]