近红外上转换纳米转换器在光遗传学调控中的应用  

Application of Near-Infrared Upconversion Nanotransducers in Optogenetic Regulation

作　　者：王志敏 胡明 邢本刚 WANG Zhi-Min;HU Ming;XING Ben-Gang(Division of Chemistry and Biological Chemistry,School of Physical&Mathematical Sciences,Nanyang Technological University,Singapore 637371,Singapore)

机构地区：[1]新加坡南洋理工大学,数理与科学学院,化学与生物化学系,新加坡637371

出　　处：《无机化学学报》2020年第6期969-982,共14页Chinese Journal of Inorganic Chemistry

基　　金：新加坡教育部及南洋理工大学Tier 1 RG5/18(S),SPMS-M4082042.110,MOE 2017-T2-2-110,A*Star SERC A1983c0028(M4070319);国家自然科学基金(No.51929201)资助项目。

摘　　要：光遗传学作为一种新兴的生物技术,能够在时间和空间上精准调控生理功能。尤其是在基于视紫红质离子通道蛋白来操控神经兴奋性及钙信号通路激活等方面,近年来该技术吸引了广泛的关注。然而,目前该技术所使用的光遗传学工具只能被可见光激发,难以穿透深层组织并实现无创地光学调控。为了解决这个问题,最近一些研究通过使用稀土掺杂上转换纳米粒子作为光转换器,将组织可穿透的近红外光转化为可见光发射,从而使复杂活体条件下的光遗传学调控成为可能。我们对近年来上转换纳米粒子介导的光遗传学技术的开发和应用进展做了详细的总结。另外,关于未来如何进一步推进该技术可用于临床研究提出了建议和展望。As an emerging biotechnology optogenetics attracts tremendous attention in spatiotemporal control of biological functions,especially in neuronal excitability manipulation through channelrhodopsins(ChRs).However,it remains a major challenge for noninvasive deep-tissue applications due to current optogenetic tools are mainly visible light-sensitive.To address this issue,recent studies have utilized lanthanide-doped upconversion nanoparticles(UCNPs),as promising photo-nanotransducers that can convert near-infrared(NIR)light to visible emissions,thereby facilitating the optogenetic regulation in complex living systems.In this review,the development and advances in UCNPs-based optogentics are summarized in detail.In addition,future perspectives for advancing such a hybrid upconversion optogenetic technique into clinical research are proposed.

关 键 词：光遗传学 上转换纳米粒子 近红外光转换器 光调控 离子通道 生理活动 

分 类 号：TN383[电子电信—物理电子学]