用于活体和细胞内SO2衍生物检测的线粒体靶向双光子磷光铱(Ⅲ)配合物  

Mitochondria-Targeted Iridium(Ⅲ)Complex Used as a Two-Photon Phosphorescent Probe for SO2 Derivatives Detection in Vitro and in Vivo

作　　者：赵子建 侯明萱 王康男 刘柳宜 毛宗万 ZHAO Zi-Jian;HOU Ming-Xuan;WANG Kang-Nan;LIU Liu-Yi;MAO Zong-Wan(MOE Key Laboratory of Bioinorganic and Synthetic Chemistry,School of Chemistry,Sun Yat-sen University,Guangzhou 510275,China)

机构地区：[1]中山大学化学学院,生物无机与合成化学教育部重点实验室,广州510275

出　　处：《无机化学学报》2020年第6期1113-1122,共10页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.21837006);教育部创新团队(IRT-17R111);中央高校基本科研业务费专项资金;中国博士后科学基金(No.2019M662968);广东省基础与应用基础研究基金联合基金(No.2019A1515110356);广东省大学生科技创新培育专项资金(No.pdjha0005)资助项目。

摘　　要：暴露于高剂量的二氧化硫(SO2)及其衍生物(SO32-和HSO3-)会导致血管疾病甚至肺癌发生。双光子磷光成像显微术(TPPIM)和双光子磷光寿命成像显微术(TPPLIM)具有良好的时空分辨能力、抗光漂白、抗自体荧光以及较强组织穿透性等优点,可以实现SO2衍生物在生物样品中实时检测。得益于铱配合物的长磷光寿命(~110 ns)和线粒体靶向特性,本文报道了首例基于TPPLIM技术的SO2衍生物检测探针Ir-EA。Ir-EA对水溶液中的亚硫酸氢盐表现出高特异性和灵敏性的识别能力(69倍磷光增强,10倍亚硫酸氢盐)和较低的检测限(65 nmol·L-1)。更为重要的是,Ir-EA对活细胞和斑马鱼的线粒体中SO2衍生物表现出良好的成像效果。Exposure to high doses of sulfur dioxide(SO2)derivatives(SO32-and HSO3-)are associated with many cardiovascular diseases and lung cancer.Benefiting from the advantages of two-photon phosphorescence imaging microscopy(TPPIM)even two-photon phosphorescence lifetime imaging microscopy(TPPLIM),including excellent spatial and temporal resolutions,anti-photobleaching,anti-autofluorescence,and strong penetration,could be promising features for real-time detection of biological bisulfite derivatives in living tissues.Herein,we firstly report the probe(named as Ir-EA)for SO2derivatives based on TPPLIM technology.Ir-EA was developed with an iridium complex according to its long phosphorescence lifetime(~110 ns)and the mitochondrial targeting properties.More importantly,Ir-EA displayed a significant luminescence enhancement(69-fold,10 eq.)and a low detection limit(65 nmol·L-1)for bisulfite in an aqueous solution.Additionally,Ir-EA can selectively lightingup mitochondria bisulfite derivatives in living cells as well as zebrafish.CCDC:1914016,Ir-EA.

关 键 词：二氧化硫衍生物 双光子磷光寿命成像 铱配合物 线粒体靶向 斑马鱼 

分 类 号：O614.82[理学—无机化学]