基于DNA和限制性核酸内切酶的基本逻辑门设计  

Basic Logic Gates Design Based on DNA and Restriction Endonuclease

作　　者：柳娟 谢文彬 汪改英 汤敏丽 LIU Juan;XIE Wenbin;WANG Gaiying;TANG Minli(School of Aerospace Engineering,Xiamen University,Xiamen 361102,China;School of Informatics,Xiamen University,Xiamen 361005,China;Shenzhen Research Institute,Xiamen University,Shenzhen 518057,China)

机构地区：[1]厦门大学航空航天学院,厦门361102 [2]厦门大学信息学院,厦门361005 [3]厦门大学深圳研究院,深圳518057

出　　处：《电子与信息学报》2020年第6期1332-1339,共8页Journal of Electronics & Information Technology

基　　金：国家自然科学基金(61772441,61872309);国家重点研发计划政府间专项(2017YFE0130600)。

摘　　要：由于DNA分子具有特异性、高并行性、微小性等天然特性,在信息处理过程中展现出了强大的并行计算能力和数据存储能力。该文研究将具有特异性识别功能的限制性核酸内切酶引入DNA链置换反应中,作为DNA电路的输入,通过控制立足点的生成和移除设计了是门、非门和与门3种基本逻辑门。采用Visual DSD对逻辑模型进行模拟仿真,并通过凝胶电泳实验验证设计。与以往的分子逻辑门比较,该设计反应迅速,操作简便,具有良好的扩展性,为大规模电路的设计提供了可能性。Due to the natural characteristics of specificity,high parallelism and miniaturization of DNA molecules,it exhibits strong parallel computing power and data storage capability in information processing.In this study,restriction endonuclease with specific recognition function are introduced into DNA strand displacement as the input of the DNA circuit.The YES gate,NOT gate and AND gate are designed by controlling the generation and removal of the toehold.The logic model is simulated by Visual DSD,and the design is verified by PolyacrylAmide Gel Electrophoresis(PAGE)experiments.Compared with previous molecular logic gates,this design has a quick response,simple operation,and good scalability,which provides the possibility for the design of large-scale circuits.

关 键 词：分子逻辑计算 DNA链置换 限制性核酸内切酶 基本逻辑门 

分 类 号：TP301[自动化与计算机技术—计算机系统结构]