8周有氧运动对小鼠骨骼肌microRNA表达的影响及其生物信息学分析  

Effects of 8-week aerobic exercise on microRNA expression in skeletal muscle of mice and its bioinformatics analysis

作　　者：梅涛 王建设[2] 张缨[3] MEI Tao;WANG Jianshe;ZHANG Ying(China Institute of Sport and Health Science,Beijing Sport University, Beijing 100084, China;Physical Education Institute,Henan Normal University, Xinxiang 453007, China;Sport Science College,Beijing Sport University, Beijing 100084, China)

机构地区：[1]北京体育大学中国运动与健康研究院,北京100084 [2]河南师范大学体育学院,河南新乡453007 [3]北京体育大学运动人体科学学院,北京100084

出　　处：《山东体育学院学报》2020年第2期89-96,共8页Journal of Shandong Sport University

基　　金：国家重点研发计划项目(2018YFF0300402);国家自然基金(31471134);中央高校基本科研业务费专项资金资助课题(2016SYS002)。

摘　　要：目的:研究8周有氧运动对骨骼肌microRNA表达的影响,并对差异表达的miRNA进行生物信息学分析以发现其可能作用途径,为后续深入研究运动对骨骼肌产生影响的机制提供方向。研究方法:20只8周龄C57BL/6J小鼠随机分为安静组(C)和运动组(E)。运动组预适应训练一周后,采用坡度0°、12 m/min、60 min/天、5天/周、持续8周的有氧训练方案进行跑台训练。于训练48小时后采集小鼠腿部骨骼肌,并提取总RNA,采用miScript miRNA PCR Arrays检测骨骼肌miRNA的表达;采用DIANA-microT-CDS、TargetScan对差异表达的miRNA进行靶基因预测,Cytoscape Bingo工具对靶基因进行Gene Ontology分析(GO分析),mirPath v.3工具对差异表达的miRNA进行通路分析。研究结果:1)共发现58个有氧运动影响骨骼肌差异表达的miRNAs,其中上调表达的miRNAs有miR-411-5p、miR-340-5p、miR-148b-3p等,共计44个;下调表达的miRNAs共有miR-709、miR-1947-3p、miR-667-5p等14个。2)靶基因预测结果显示,上调表达的miRNA共有9122个靶基因,下调表达的miRNAs共有3636个靶基因。3)GO分析发现,上调表达的miRNA的靶基因GO功能富集主要包括核酸结合、蛋白质结合、DNA结合、调节基因表达、大分子代谢过程等;下调表达的miRNA的靶基因GO功能富集主要包括核酸结合、蛋白质结合、核酸代谢过程、转录过程等。4)miRNA通路分析结果显示,上调表达的miRNA靶向的通路有MAPK信号通路、mTOR信号通路、破骨细胞分化等;下调表达的miRNA靶向的通路有糖胺聚糖生物合成-硫酸软骨素信号通路、Wnt信号通路、MAPK信号通路等。结论:有氧运动能够引起骨骼肌内miRNA的差异表达;差异表达miRNAs可能通过靶向MAPK信号通路、Wnt信号通路和mTOR信号通路等调控骨骼肌的生长发育和生理生化过程。Objective:To analyze the functions of miRNAs which were affected by 8-week aerobic exercise in skeletal muscle of mice.Methods:Twenty C57BL/6J mice were randomly divided into exercise(E)and control(C)groups.The E group were trained on a treadmill with a program of slope 0°,12m/min,60min/day,and 5 days/week.The C group lived normally without training.After 8-week of training,skeletal muscles were harvested for RNA extraction.Subsequently,miScript miRNA PCR Arrays was conducted to identify the differentially expressed miRNAs between two groups.Target gene prediction was performed by using TargetScan and microT-CDS tools.Gene Ontology analysis(GO)on the target gene was performed with Cytoscape Bingo.MirPath v.3 from the DIANA tools was used to execute the Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses.Results:A total of 58 miRNAs affecting the differential expression of skeletal muscle were found.44 miRNAs were upregulated(miR-411-5p,miR-340-5p,miR-148b-3p,etc.)and 14 miRNAs were downregulated(miR-709,miR-1947-3p,miR-667-5p,etc.)after the aerobic exercise.Prediction of target genes showed that there were a total of 9122 target genes for the up-regulated miRNAs,while 3636 target genes for the down-regulated miRNAs.GO analysis revealed that the differentially expressed miRNAs participated in a variety of molecular functions and biological processes including nucleic acid binding,protein binding,regulation of gene expression,macromolecule metabolic process and others.KEGG analysis showed that the up-regulated miRNAs were significantly enriched in MAPK,Wnt and mTOR signaling pathway.The down-regulated miRNAs were mainly enriched in MAPK,Wnt signaling pathway.Conclusion:Differential expression of miRNAs in skeletal muscle could be induced by Aerobic exercise.The physiological and biochemical processes may be regulated by the differentially expressed miRNAs in skeletal muscle by targeting MAPK signaling pathway,Wnt signaling pathway and mTOR signaling pathway,which provide directions for further research.

关 键 词：有氧运动 MICRORNA 骨骼肌 生物信息学分析 

分 类 号：G804.2[文化科学—运动人体科学]