艾滋病患者感染新型隐球菌的MLST分型及临床特点分析  

MLST Typing and Clinical Characteristics of New Cryptococcus Infections in AIDS Patients

作　　者：徐新民[1] 张媛媛[2] 杜鹏程[2] 李敏[1] 王慧珠[1] 王雅杰 XU Xinmin;ZHANG Yuanyuan;DU Pengcheng;LI Min;WANG Huizhu;WANG Yajie(Department of Clinical Laboratory,Beijing Ditan Hospital,Capital Medical University,Beijing 100015;Institute of Infectious Disease,Beijing Ditan Hospital,Capital Medical University,Beijing 100015)

机构地区：[1]首都医科大学附属北京地坛医院检验科,北京100015 [2]首都医科大学附属北京地坛医院传染病研究所,北京100015

出　　处：《标记免疫分析与临床》2020年第5期829-832,共4页Labeled Immunoassays and Clinical Medicine

基　　金：新发突发传染病研究北京市重点实验室开放课题(编号:DTKF201801)。

摘　　要：目的了解艾滋病患者感染新型隐球菌的基因分型情况。方法收集我院50例艾滋病合并新型隐球菌感染患者的分离菌株,采用PCR测序的方法对分离的新型隐球菌的7个管家基因(CAP59、GPD1、LAC1、PLB1、SOD1、URA5和IGS1)进行PCR扩增和测序,将序列上传至Fungal MLST Database得到序列类型(sequence type,ST),最后通过BioNumerics 4.0软件对ST分型结果进行最小生成树分析。结果50例艾滋病患者中,88.0%(44/50)的为男性,其中31~50岁之间患者占56.0%(28/50),60%患者有中枢神经系统感染,从50例患者的不同部位分离出53株新型隐球菌,其MLST分型分析结果显示,共分离出9种基因型,81.1%(43/53)的菌株为ST5型,ST31和ST296分别有2株,ST63、ST289、ST295、ST298、ST324和ST337分别有1株。结论艾滋病患者感染新型隐球菌以男性为主,北京地区MLST基因分型主要是ST5型,为新型隐球菌的研究提供分子流行病学依据。Objective To understand the genotyping of Cryptococcus neoformans in HIV patients.Methods We collected isolates of 50 patients with AIDS and Cryptococcus neoformans infection from our hospital,and used PCR-sequencing method to perform PCR amplification and sequencing for seven housekeeping genes(CAP59,GPD1,LAC1,PLB1,SOD1,URA5 and IGS1)of Cryptococcus neoformans,then uploaded the sequence to Fungal MLST database to obtain the sequence type(ST),and finally perform the minimum spanning tree analysis on the ST typing results by BioNumerics 4.0 software.Results Of the 50 AIDS patients,88.0%(44/50)were male,56.0%(28/50)were between 31 and 50 years old,and 60%of patients had central nervous system infections.A total of 53 strains of Cryptococcus neoformans were isolated from different specimens of 50 patients.The MLST typing analysis showed that 9 genotypes were isolated,in which 81.1%(43/53)of the strains were ST5 type,also ST31 and ST296 had 2 strains,and ST63,ST289,ST295,ST298,ST324 and ST337 had 1 strain,respectively.Conclusion The majority of new Cryptococcus infections in AIDS patients are male,and the MLST genotyping is mainly ST5 type,which provides a molecular epidemiological basis for the research of newly developed Cryptococcus.

关 键 词：艾滋病 新型隐球菌 MLST分型 

分 类 号：R512.91[医药卫生—内科学]