不同程度心理应激对小鼠HPA轴及卵巢储备功能的影响  

Different Degrees of Psychological Stress on Stress Hormone Level of HPA Axis and Ovarian Reserve Function in Mice

作　　者：刘妍 程蕊 孙若芸 马瑞红[1] 夏天[1] LIU Yan;CHENG Rui;SUN Ruo-yun;MA Rui-hong;XIA Tian(Reproductive Center,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China)

机构地区：[1]天津中医药大学第一附属医院生殖中心,300193

出　　处：《国际妇产科学杂志》2020年第3期311-314,I0001,共5页Journal of International Obstetrics and Gynecology

基　　金：国家自然科学基金(81603650)。

摘　　要：目的:探讨不同程度心理应激对小鼠下丘脑-垂体-肾上腺(HPA)轴中应激激素水平以及卵巢储备功能的影响。方法:将80只4~5周龄雌性未孕小鼠随机分为4组,每组20只,建立不同程度心理应激模型,4周后取其内眦静脉丛血检测小鼠HPA轴应激激素水平及性激素水平,取其卵巢组织制作石蜡切片,光镜下观察卵巢组织学形态变化。结果:经不同程度刺激4周后,4组应激激素[促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、皮质酮(CORT)]和性激素[卵泡刺激素(FSH)、黄体生成激素(LH)、雌二醇(E2)、抗苗勒管激素(AMH)]水平比较,差异均有统计学意义(P<0.05)。随着心理应激程度的增强,小鼠血清应激激素水平逐渐升高(Ptrend<0.01),性激素水平逐渐降低(Ptrend<0.01)。心理应激程度越强,光镜下各级生长卵泡越少,闭锁卵泡越多。结论:心理应激程度越强,HPA轴功能越亢奋,对HPO轴抑制越明显,使小鼠性激素分泌紊乱,卵泡发育异常,导致卵巢储备功能下降,从而影响其生殖功能。Objective:To investigate the changes of the related indexes of the serological related different degrees of psychological stress,the changes of the serum sex hormone levels in mice and the morphological changes of the ovaries under light microscope by establishing the model of psychological stress in mice.Methods:A total of 80 SPF grade healthy female ICR mice with a age of 4-5 weeks were collected.They were randomly divided into 4 groups,20 in each group,giving different degrees of stimulation in 4 weeks,making the animal models of the psychological stress mice.The ovarian tissue was taken to make paraffin sections,and the histological and morphological changes of ovary were observed under light microscope.Result:After 4 weeks of stimulation,the level of 4 groups of stress hormones[corticotrophin releasing hormone(CRH),adrenocorticotropic hormone(ACTH),corticosterone(CORT)]and sex hormones[follicle stimulating hormone(FSH),luteinizing hormone(LH),estradiol(E2),anti-Mullerian hormone(AMH)]was statistically significant(P<0.05).With the increase of psychological stress,the levels of serum stress hormones in mice increased gradually(Ptrend<0.01),and the levels of sex hormones gradually decreased(Ptrend<0.01).The stronger the degree of psychological stress,the less the growth follicles at all levels under the light microscope,and the more the follicles are blocked.Conclusions:The stronger the level of psychological stress,the more active the HPA axis,and the more obvious the inhibition of the HPO axis,which makes the sex hormone secretion in mice and abnormal follicular development,resulting in decreased ovarian reserve function,which affects its reproductive function.

关 键 词：应激 心理学 下丘脑-垂体系统 激素类 性腺甾类激素 卵巢储备功能 

分 类 号：R711[医药卫生—妇产科学]