鸢尾素后处理对大鼠肝缺血再灌注的影响  

Effect of irisin postconditioning on hepatic ischemia reperfusion injury in rats

作　　者：杨新[1] 吴会生[1] 王华桥 杨铁成 王丹雯 杨丽洁 冯茂辉[2] 王焱林[1] Yang Xin;Wu Huisheng;Wang Huaqiao;Yang Tiecheng;Wang Danwen;Yang Lijie;Feng Maohui;Wang Yanlin(Department of Anesthesiology,Zhongnan Hospital,Wuhan University,Wuhan 430071,China;Department of Gastrointestinal Surgery,Zhongnan Hospital,Wuhan University,The Clinical Medical Research Center of Peritoneal Cancer of Wuhan,Clinical Cancer Study Center of Hubei Provence,Key Laboratory of Tumor Biological Behavior of Hubei Provence,Wuhan 430071,China)

机构地区：[1]武汉大学中南医院麻醉科,430071 [2]武汉大学中南医院胃肠外科,武汉市腹膜癌临床医学研究中心,湖北省肿瘤医学临床研究中心,肿瘤生物学行为湖北省重点实验室,430071

出　　处：《中华实验外科杂志》2020年第1期56-59,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(81770283);武汉市腹膜癌临床医学研究中心资助项目(2015060911020462)。

摘　　要：目的观察鸢尾素后处理对大鼠肝缺血再灌注损伤的影响并探讨其机制。方法健康雄性SD大鼠(武汉大学实验动物中心提供)36只,采用随机数字表法分为假手术组(S组)、缺血再灌注组(IR组)和鸢尾素组(Ir组),每组12只。IR组和Ir组大鼠采用夹闭左、中叶肝蒂建立大鼠肝脏70%缺血再灌注模型(缺血60 min,再灌注6 h);Ir组于灌注开始时静脉注射鸢尾素10μg/kg;S组仅游离肝脏周围血管及韧带。再灌注6 h后下腔静脉采血并留取肝脏组织标本。检测血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、白细胞介素(IL)-6、IL-1β、肿瘤坏死因子-α(TNF-α)含量及肝脏组织丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX)含量;苏木精-伊红(HE)染色,光镜观察肝脏组织病理学的变化。用蛋白质印迹法检测磷酸化信号转导与转录激活因子3(p-STAT3)和磷酸化Janus激酶2(p-JAK2)的表达水平及B细胞淋巴瘤/白血病-2(bcl-2)、bcl-2相关X蛋白(bax)、半胱氨酰天冬氨酸特异性蛋白酶(Caspase)-3含量。采用SPSS软件对3组大鼠肝脏指标进行方差分析,并进行两两比较。结果与S组比较,IR组肝脏组织病理学损伤较重;S组、I/R组和Ir组的ALT含量分别为(75.24±2.65)、(705.33±4.02)、(385.46±3.58)U/L;AST含量分别为(122.33±6.76)、(1357.54±5.23)、(738.26±3.98)U/L;IL-6含量分别为(104±16)、(586±86)、(275±35)ng/L;TNF-α含量分别为(92±10)、(1165±102)、(511±73)ng/L;IL-1β含量分别为(98±12)、(610±79)、(312±41)ng/L;MDA含量分别为(174±38)、(1900±460)、(1055±266)ng/L;SOD含量分别为(124±10)、(46±8)、(70±10)ng/L;GPX含量分别为(106±12)、(42±5)、(62±11)ng/L;Caspase-3含量分别为(0.22±0.06)、(0.86±0.14)、(0.57±0.11)ng/L;p-JAK2含量分别为0.44±0.05、0.91±0.07、0.62±0.11;p-STAT3含量分别为0.35±0.04、0.86±0.08、0.57±0.07。I/R组及Ir组大鼠血清ALT、AST、IL-6、TNF-α、IL-1β及肝组织MDA、Caspase-3升高(t=445.Objective To investigate the effect and mechanism of irisin postconditioning on hepatic ischemia reperfusion injury in rats.Methods Totally 36 SD rats were randomly divided into sham group(S),model group(I/R)and irisin group(Ir),with 12 rats in each group.Hepatic ischemia was constructed in model group and irisin group by ligating left middle lobe of liver vascular branches for 60 min.Irisin was administered at the beginning of reperfusion by intravenous injection at the concentration of 10μg/kg for rats in irisin group.We only dissected the hepatic duodenal ligament of rats in the S group.Serum and liver tissues were collected at 6 h after reperfusion through the inferior chamber.The serum levels of alanine aminotransferase(ALT),glutamic oxalacetic transaminase(AST),interleukin(IL)-6,IL-1βand tumor necrosis factor-α(TNF-α)were examined.The contents of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase(GPX)in liver tissue were measured.The pathological changes of liver tissue were observed by hematoxylin and eosin(HE)staining.The phosphorylations of janus kinase 2(JAK2),signal transducer and activators of transcription 3(STAT3)and B cell lymphoma/leukemia-2(bcl-2),bcl-2 associated X protein(bax),cysteinyl aspartate-specific protease(Caspase)-3 were assessed by Western blotting.Results The ALT levels in S group,I/R group and irisin group were as follows:(75.24±2.65),(705.33±4.02),(385.46±3.58)U/L;The AST levels were as follows:(122.33±6.76),(1357.54±5.23),(738.26±3.98)U/L;The IL-6 levels were as follows:(104±16),(586±86),(275±35)ng/L;The TNF-αlevels were as follows:(92±10),(1165±102),(511±73)ng/L;The IL-1βlevels were as follows:(98±12),(610±79),(312±41)ng/L;The MDA contents were as follows:(174±38),(1900±460),(1055±266)ng/L;The SOD contents were as follows:(124±10),(46±8),(70±10)ng/L;The GPX contents were as follows:(106±12),(42±5),(62±11)ng/L;The Caspase-3 levels were as follows:(0.22±0.06),(0.86±0.14),(0.57±0.11)ng/L;The p-JAK2 levels were as follows:0.44±0

关 键 词：鸢尾素 肝缺血 再灌注损伤 JANUS激酶2 信号转录与转录激活因子3 

分 类 号：R28[医药卫生—中药学]