机械振动对去卵巢大鼠骨折愈合中成纤维细胞生长因子23抗体的调节作用  

Regulatory effect of mechanical vibration on fibroblast growth factor 23 antibody during fracture healing in ovariectomized rats

作　　者：汪学红[1] 孙大明[1] 周丹丹 李婷婷[1] 王艺喆 洪勇良 程凯[3] Wang Xuehong;Sun Daming;Zhou Dandan;Li Tingting;Wang Yizhe;Hong Yongliang;Cheng Kai(Wuhan Institute of Physical Education,Wuhan 430079,China;Hunan University of Chinese Medicine,Hunan 410208,China;Department of Neurosurgery,Liyuan Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430077,China)

机构地区：[1]武汉体育学院,430079 [2]湖南中医药大学,长沙410208 [3]华中科技大学同济医学院附属梨园医院神经外科,武汉430077

出　　处：《中华实验外科杂志》2020年第1期118-120,共3页Chinese Journal of Experimental Surgery

基　　金：湖北省自然科学基金(2011CDB312);武汉体育学院青年教师科研基金资助项目(2014QZ09)。

摘　　要：目的探讨机械振动干预对去卵巢骨折大鼠内分泌激素成纤维细胞生长因子23(FGF23)的调控作用。方法45只3月龄Wistar雌性大鼠(湖北省疾病控制中心提供)制作绝经后骨质疏松模型,随机分为假去卵巢骨折对照组(Sham)、去卵巢骨折模型组(OVX)和去卵巢骨折振动组(OVX-V),每组15只。OVX-V组大鼠骨折术后5 d实施频率为35 Hz,振动幅度为2 mm,加速度为0.5 g的全身振动,20 min/d,5天/周。Sham组和OVX组在术后5 d放在振动台上活动20 min,不进行振动。使用蛋白质印迹法(Western blot)检测大鼠骨折端FGF23蛋白的表达。应用SPSS 19.0统计软件分析,计量资料以均值±标准差(Mean±SD)表示,组间比较采用单因素方差分析。结果大鼠骨折端FGF23蛋白的表达,OVX-V组骨折端FGF23蛋白表达在第2、4、6周(0.703±0.009、0.466±0.011、0.380±0.005)均显著低于OVX组(0.846±0.012、1.315±0.021、1.105±0.014),差异有统计学意义(P<0.01),呈下降趋势;OVX组骨折端FGF23蛋白表达在第2、4、6周(0.846±0.012、1.315±0.021、1.105±0.014)均高于Sham组(0.168±0.004、0.321±0.003、0.417±0.009),,差异有统计学意义(P<0.01),呈上升趋势;提示机械振动能降低去卵巢大鼠骨组织FGF23表达量。结论机械振动可以降低骨组织中FGF23的表达,促进成骨细胞分化,调节骨矿化,促进绝经后骨质疏松后骨折愈合。Objective To investigate the regulatory effect of mechanical vibration intervention on endocrine hormone fibroblast growth factor23(FGF23)in ovariectomized fracture rats.Methods A model of postmenopausal osteoporosis was established in 45 female rats,and the animals were randomly divided into three groups:Sham operation group(Sham,n=15),ovariectomized fracture model group(OVX,n=15)and ovariectomized fracture vibration group(OVX-V,n=15).The rats in OVX-V group were subjected to whole-body vibration at a frequency of 35 Hz,a vibration amplitude of 2 mm,and an acceleration of 0.5 g,20 min/d and 5 t/w,5 days after fracture surgery.The rats in Sham group and OVX group were placed on the vibrating table for 20 min at 5th day after the operation,and no vibration was performed.Western blotting was used to detect the expression of FGF23 protein in the fracture end of rats.SPSS 19.0 statistical software was used to analyze the results.The results were expressed as mean±standard deviation(Mean±SD).Comparisons between groups were analyzed by one-way ANOVA.Results The protein expression of FGF23 in the fracture end of OVX-V group was significantly lower than that in OVX group at 2nd,4th and 6th week[(0.846±0.012,1.315±0.021,1.315±0.021)vs.(0.703±0.009,0.466±0.011,0.380±0.005),P<0.01]..The protein expression of FGF23 in the fracture end of OVX group was significantly higher than that in Sham group at 2nd,4th and 6th week[(0.168±0.004,0.321±0.003,0.417±0.009)vs.(0.846±0.012,1.315±0.021,1.315±0.021),P<0.01],showing an increasing trend,suggesting that mechanical vibration can reduce the expression level of FGF23 in the bone tissue of oarotomy rats.Conclusion Mechanical vibration can reduce the expression of FGF23 in bone tissue,promote osteoblast differentiation,regulate bone mineralization and promote postmenopausal osteoporosis fracture healing.

关 键 词：机械振动 骨质疏松 骨折 内分泌激素 成纤维细胞生长因子23 

分 类 号：R6[医药卫生—外科学] R5[医药卫生—临床医学]