当归四逆汤对糖尿病大鼠坐骨神经miR-210、HIF-1α和VEGF表达的影响  

Effects of The Chinese Herbal Medicine Prescription Danggui Sini Decoction for Frigid Extremities on Mir-210,HIF-1αand VEGF Expression of Sciatic Neuropathy in Streptozotocin-induced Diabetic Rats

作　　者：胡文梅[1] 贾爱明[2] 白长川 HU Wen-mei;JIA Ai-ming;BAI Chang-chuan(ZhongShan College of Dalian Medical University,Liaoning,Dalian 116085,China;Second Affiliated Hospital of Dalian Medical University,Liaoning,Dalian 116023,China;Dalian Hospital of Traditional Chinese Medicine,Liaoning,Dalian 116013,China)

机构地区：[1]大连医科大学中山学院实验中心,辽宁大连116085 [2]大连医科大学附属第二医院,辽宁大连116023 [3]大连市中医医院,辽宁大连116013

出　　处：《中国中医基础医学杂志》2020年第5期605-608,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：第四批全国中医(临床、基础)优秀人才研修项目(国中医药人教发[2017]24号);全国名中医传承工作室建设项目(国中医药办人教函[2018]119号);大连市中医药相关科研计划项目(2016Z2007)-基于“孙络-微血管”理论探讨当归四逆汤干预糖尿病周围神经病变机制。

摘　　要：目的:探讨当归四逆汤对糖尿病大鼠坐骨神经miR-210、HIF-1α和VEGF表达的影响。方法:30只SD大鼠分为空白对照组和观察组,观察组采用1%链脲佐菌素50 mg/kg诱导生成糖尿病大鼠模型,造模成功的观察组大鼠17只按随机数字表法分为糖尿病模型组和中药预防组。MP组灌服当归四逆汤(12 mL/kg/d),其余组灌服生理盐水,12周结束后,检测3组大鼠空腹血糖、光热刺激痛阈时间及坐骨神经传导速度,并取坐骨神经检测miR-210、HIF-1αmRNA水平及VEGF表达。结果:12周后与BC组比较,DM组空腹血糖、光热刺激痛阈时间明显升高,坐骨神经传导速度、miR-210、HIF-1αmRNA及VEGF表达明显降低,差异有统计学意义。与DM组比较,MP组大鼠光热刺激痛阈时间明显降低,坐骨神经传导速度、miR-210、HIF-1αmRNA及VEGF表达明显增高,差异有统计学意义。结论:早期预防性应用当归四逆汤可改善糖尿病周围神经病变,其机制可能与上调神经组织miR-210、HIF-1αmRNA及VEGF表达有关。Objective:To observe the effects of the Chinese Herbal Medicine Prescription Danggui Sini Decoction for Frigid Extremities(DDFE)on miR-210,HIF-1αand VEGF expression of Sciatic Neuropathy in Streptozotocin-Induced Diabetic Rats.Methods:30 SD rats were randomly assigned to two groups:the observation group(n=22)and blank control group(BC group,n=8).The observation group received a single intraperitoneal injection of 1%streptozotocin(50mg/kg body weight)to prepare the model group of Diabetic Rats.17 rats after successful modeling in the observation group were randomly divided into diabetic model group(DM group,n=8)and medicine prophylaxis group(MP group,n=9).The DDFE at a dosage of 12 mL/kg/d was administered to the rats in the MP group,while the other rats were given distilled water at the same dosage.Fasting blood glucoses,thermal thresholds,sciatic nerve conduction velocities,and the levels of miR-210,HIF-1 mRNA and VEGF protein in sciatic nerves were measured after 12 weeks.Results:Fasting blood glucoses,thermal thresholds were significantly increased while sciatic nerve conduction velocities,levels of miR-210,HIF-1 mRNA and VEGF protein were significantly decreased compared with BC group 12 weeks later.Thermal thresholds were significantly decreased while sciatic nerve conduction velocities,levels of miR-210,HIF-1 mRNA and VEGF protein were significantly increased compared with DM group after 12 weeks of treatment with DDFE.Conclusion:Early preventive application of DDFE may delay the development of DPN via increasing miR-210,HIF-1αmRNA and VEGF expression in the sciatic nerves of diabetic rats.

关 键 词：当归四逆汤 糖尿病周围神经病变 MIR-210 缺氧诱导因子-1Α 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]