低钙高镁人工脑脊液对大鼠脑片臂旁外侧核神经元温度敏感性的影响  

Effects of low Ca^2+ high Mg^2+ artificial cerebrospinal fluid on the thermosensitivity of lateral parabrachial neurons in rat brainstem slices

作　　者：邓杰文 胡红利 杨培培 黄成 赵曦 全俊如 许莉 张录顺[1] 张洁[1] Deng Jie-wen;Hu Hong-li;Yang Pei-pei;Huang Cheng;Zhao Xi;Quan Jun-ru;Xu Li;Zhang Lu-shun;Zhang Jie(School of Basic Medical Sciences,Chengdu Medical College,Chengdu 610500,China;School of Clinical Medical Sciences,Chengdu Medical College,Chengdu 610500,China;School of Laboratory Medicine,Chengdu Medical College,Chengdu 610500,China)

机构地区：[1]成都医学院基础医学院,四川成都610500 [2]成都医学院临床医学院,四川成都610500 [3]成都医学院检验医学院,四川成都610500

出　　处：《四川生理科学杂志》2020年第2期123-128,共6页Sichuan Journal of Physiological Sciences

基　　金：国家自然科学基金面上项目(编号:31771289);四川省教育厅重点项目(编号:17ZA0112);国家级大学生创新创业训练项目(编号:201713705041)。

摘　　要：目的:探讨臂旁外侧核(Lateral parabrachial nucleus,LPBN)神经元的温度敏感性是否依赖于突触传递。方法:制备含LPBN的大鼠脑干片后,在32~40℃范围内循环改变灌流液温度,利用脑片膜片钳技术,鉴定LPBN神经元温度敏感性;然后灌流低钙高镁人工脑脊液(Ca^2+0.2 mmol·L^-1,Mg^2+11.4 mmol·L^-1)阻断突触传递,进一步检测大鼠LPBN热敏及温度不敏感神经元在不同温度下的胞外自发放电活动的变化,分析其温度敏感系数的变化。结果:阻断突触传递后,热敏神经元的自发放电活动及温度敏感系数均无显著变化(P>0.05);阻断突触传递后,中等斜率(n=11)和低等斜率(n=11)的温度不敏感神经元的自发放电频率呈多样性改变,放电幅度无明显改变(P>0.05),温度敏感系数大部分显著提高(P<0.05),其中4个神经元在突触阻断后,从温度不敏感神经元变成热敏神经元,洗脱后又恢复为温度不敏感神经元。结论:LPBN热敏神经元的温度敏感性与突触传递无关,属于内在特性。Objective:To investigate whether neuronal thermosensitivity of lateral parabrachial(LPB)neurons is dependent on synaptic transmission.Methods:The spontaneous firing activity of LPB neurons in rat brainstem slices was extracellularly monitored by loose-seal cell-attached patch clamp recordings.After identifying the thermosensitivity of LPB neurons,low Ca^2+high Mg^2+artificial cerebrospinal fluid(ACSF,Ca^2+0.2 mmol·L^-1 and Mg^2+11.4 mmol·L^-1)was perfuse to block synaptic transmission to detect the firing rate,amplitude and thermal coefficients of different types of LPB neurons.Results:During the blockade of synaptic transmission,the firing activity and thermosensitivity of the warm-sensitive neurons in the LPBN did not markedly change(P>0.05).The firing rate of the moderate-slope(n=11)and low-slope(n=11)temperature-insensitive neurons in the LPBN showed the variety of responses.The thermal coefficients were significantly increased(P<0.05)without changing with firing amplitude(P>0.05).Four of 22 temperature-insensitive neurons became warm-sensitive neurons,which recovered once regular ACSF was reinstated.Conclusion:The intrinsic thermosensitivity of warm-sensitive neurons in the LPBN didn’t depend on synaptic transmission.

关 键 词：臂旁外侧核 突触传递 温度敏感性 

分 类 号：Q42[生物学—神经生物学]