建兰花叶病毒和齿兰环斑病毒的检测技术及脱毒方法研究进展  

Cymbidium mosaic virus and Odontoglossum ringspot virus:Research Progress on Detection Techniques and Virus Elimination

作　　者：王仁睿 李杰[1] Wang Renrui;Li Jie(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang Sichuan 621010)

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010

出　　处：《中国农学通报》2020年第11期56-62,共7页Chinese Agricultural Science Bulletin

基　　金：四川省教育厅项目“洋兰分生组织超低温脱毒关键技术研究”(16zd1137);西南科技大学博士基金“兰花超低温疗法脱毒技术”(15zx7117)。

摘　　要：为了弄清目前建兰花叶病毒(CymMV)和齿兰环斑病毒(ORSV)病毒检测和脱毒的研究概况,本研究详细阐述了2种病毒的检测方法、采用的脱毒技术和脱毒效率,分析比较了不同技术存在的弊端,指出现有的脱毒方法已不能满足兰科植物脱毒研究的需要。在此基础上,进一步提出应采用安全、可靠的新型脱毒技术来解决目前兰科植物产业瓶颈问题。超低温脱毒技术是近年来发展迅速的脱毒技术,具有独特优势,目前已在多种植物上成功应用,该方法的应用有望解决2种病毒脱毒方面存在的问题。To clarify the research development of detection techniques and virus elimination methods of Cymbidium mosaic virus(CymMV) and Odontoglossum ringspot virus(ORSV), we summarized the detection and elimination methods of the two viruses and the virus elimination efficiency, analyzed the defects of traditional virus-free methods, and pointed out that the traditional virus-free methods could not meet the demand of orchid virus elimination research. Based on the analysis, a new safe and reliable virus-free method is proposed to solve the bottleneck of orchid industry. Cryotherapy, which has many advantages compared with the traditional methods, has been proven to be a new technique for plant pathogen elimination with successful application on several plants, and can provide a technical support to CymMV and ORSV elimination.

关 键 词：建兰花叶病毒 齿兰环斑病毒 病毒检测 传统脱毒方法 超低温脱毒技术 

分 类 号：S682.31[农业科学—观赏园艺]