毛细支气管炎患儿血IL-4、IL-10、IL-17的变化及意义  

Changes and significances of IL-4,IL-10 and IL-17 levels in peripheral blood in children with bronchiolitis

作　　者：朱其国 袁林[1] 林建成 童秀斌 ZHU Qiguo;YUAN Lin;LIN Jiancheng;TONG Xiubin(Children\s Hospital of Fudan University Xiamen Branch,Xiamen Children\s Hospital,Fujian Xiamen 361006,China)

机构地区：[1]复旦大学附属儿科医院厦门分院厦门市儿童医院,福建厦门361006

出　　处：《中国妇幼健康研究》2020年第5期649-652,共4页Chinese Journal of Woman and Child Health Research

基　　金：厦门市科技局科技惠民资助项目(项目编号:3502Z20164069)。

摘　　要：目的探讨呼吸道合胞病毒(RSV)引起的毛细支气管炎患儿外周血白细胞介素(IL)-4、IL-10、IL-17水平的变化及意义。方法选取2017年1至12月在厦门市儿童医院住院的38例RSV引起的毛细支气管炎患儿为研究对象(包括轻度11例、中度18例和重度9例),作为RSV毛支组,同时选取同年龄支气管肺炎患儿31例及健康体检儿童39例分别作为支气管肺炎组和健康对照组,记录各组患儿的临床资料,全部儿童均采用酶联免疫吸附法检测患儿血清细胞因子IL-4、IL-10、IL-17含量,比较各组检测结果。结果①RSV毛支组患儿的IL-4、IL-17水平高于支气管肺炎组和健康对照组,差异均有统计学意义(IL-4比较:t值分别为-3.53、-8.47;IL-17比较:t值分别为-4.96、-11.33,均P<0.05);RSV毛支组IL-10水平低于支气管肺炎组和健康对照组,差异均有统计学意义(t值分别为2.74、6.28,均P<0.05);而支气管肺炎组和健康对照组的IL-4、IL-10、IL-17水平比较,差异均无统计学意义(均P>0.05)。②重度RSV毛支患儿的IL-17水平高于轻度和中度患儿,差异有统计学意义(t值分别为5.14、8.97,均P<0.05);而三种不同程度RSV毛支患儿的IL-4、IL-10水平比较,差异均无统计学意义(均P>0.05)。结论 RSV引起的毛细支气管炎发病与细胞因子IL-4、IL-10、IL-17水平的变化密切相关,IL-17水平与RSV引起的毛细支气管炎的严重程度密切相关。Objective To observe the changes and significances of IL-4,IL-10 and IL-17 levels in peripheral blood in children with bronchiolitis caused by respiratory syncytial virus(RSV). Methods 38 children with RSV bronchiolitis admitted to Xiamen Children’s Hospital from January 2017 to November 2017 were enrolled as RSV bronchiolitis group(including 11 cases of mild,18 cases of moderate and 9 cases of severe,respectively). At the same time,31 children of the same age with bronchial pneumonia and 39 healthy children of the same age were enrolled as the pneumonia group and the control group. Clinical data of children in each group was recorded. The serum levels of IL-4,IL-10 and IL-17 were detected by enzyme-linked immunosorbent assay in all children,and the test results in each group were compared. Results The serum levels of IL-4 and IL-17 in children with RSV bronchiolitis(41.48±20.16,515.39±124.30) were significantly higher than those in the pneumonia group(31.38±13.56,410.39±144.49) and control group(27.02±13.13,357.70±136.51),the differences were statistically significant(t=-3.53,-8.47,-4.96 and-11.33,respectively,P<0.05). The serum level of IL-10 in the RSV bronchiolitis group(14.34±3.88) was significantly lower than that in the pneumonia group and control group(t=2.74 and 6.28,respectively,both P<0.05). But no significant difference was found in the serum levels of IL-4、IL-10 and IL-17 between the pneumonia group and the control group(all P>0.05). The serum level of IL-17 in children with severe RSV bronchiolitis was significantly higher than that in mild and moderate children(t= 5.14 and 8.97,respectively,both P<0.05). But no significant difference was found in the serum levels of IL-4 and IL-10 among children with different degrees of RSV(all P>0.05). Conclusion The changes of cytokine IL-4、IL-10 and IL-17 levels are closely related to the bronchiolitis caused by respiratory syncytial virus. And the severity of RSV bronchiolitis is closely related to the serum level of IL-17.

关 键 词：呼吸道合胞病毒 毛细支气管炎 白细胞介素-4 白细胞介素-10 白细胞介素-17 

分 类 号：R725.6[医药卫生—儿科]