人参皂苷Rg1对非酒精性脂肪肝HepG2细胞脂质摄取和氧化的影响  

Effect of Ginsenoside Rg1 on Lipid Uptake and Oxidation in HepG2 Cells of Nonalcoholic Fatty Liver Disease

作　　者：高月 黄文祥[1] 章述军[1] 李佳俊[1] 阳成[1] 赵金秋[1] 肖晴 杨福伟 朱雅莉 GAO Yue;HUANG Wen-xiang;ZHANG Shu-jun;LI Jia-jun;YANG Cheng;ZHAO Jin-qiu;XIAO Qing;YANG Fu-wei;ZHU Ya-li(The First Affiliated Hospital of Chongqing Medical University.Chongqing 400016,China)

机构地区：[1]重庆医科大学附属第一医院,重庆400016

出　　处：《中国实验方剂学杂志》2020年第12期100-106,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：重庆市科委基础科学与前沿技术研究重点项目(CSTC2015jcyjBX0079)。

摘　　要：目的:探讨人参皂苷Rg1改善游离脂肪酸(FFA)诱导的非酒精性脂肪肝(NAFLD)HepG2细胞脂质摄取和氧化的作用及机制。方法:将HepG2细胞分为空白组,模型组,人参皂苷Rg1低、高剂量组(25,50μmol·L^-1)。1 mmol·L^-1游离脂肪酸处理HepG2细胞24 h构建非酒精性脂肪肝细胞模型,25,50μmol·L^-1人参皂苷Rg1处理24 h。细胞增殖及毒性检测-8(CCK-8)检测人参皂苷Rg1对HepG2细胞活性的影响,微量法检测细胞内甘油三酯(TG)含量,油红O染色观察脂滴聚集情况,实时荧光定量聚合酶链式反应(Real-time PCR)及蛋白免疫印迹法(Western blot)检测与脂质摄取和氧化相关基因与蛋白的表达情况。结果:与空白组比较,模型组细胞内TG含量及脂滴聚集吸光度显著增加(P<0.01);与模型组比较,人参皂苷Rg1组细胞内TG含量及脂滴聚集吸光度显著降低(P<0.01)。Real-time PCR和Western blot结果均显示,与空白组比较,模型组过氧化物酶体增殖物激活受体γ(PPARγ),脂肪酸结合蛋白1(FABP1),脂肪酸转运蛋白2/5(FATP2/5)和脂肪酸转运酶(CD36)mRNA和蛋白的表达明显升高(P<0.05),过氧化物酶体增殖物激活受体α(PPARα),肉毒碱棕榈酰基转移酶1(CPT1)和酰基辅酶A氧化酶1(ACOX1)mRNA和蛋白的表达明显降低(P<0.05);与模型组比较,人参皂苷Rg1组PPARγ,FABP1,FATP2,FATP5和CD36mRNA和蛋白的表达明显降低(P<0.05,P<0.01),PPARα,CPT1和ACOX1 mRNA和蛋白的表达明显升高(P<0.05,P<0.01)。结论:人参皂苷Rg1可通过降低脂质摄取和增强脂质氧化减少NAFLD细胞模型脂质蓄积。Objective:To investigate the effect and mechanism of ginsenoside Rg1(G-Rg1)in ameliorating lipid uptake and oxidation in HepG2 cells induced by free fatty acids(FFA).Method:HepG2 cells were divided into normal group,model group,low-dose ginsenoside Rg1 group(25μmol·L^-1)and high-dose GRg1 group(50μmol·L^-1).HepG2 cells were treated with 1 mmol·L^-1 free fatty acid for 24 h to construct the NAFLD cell model,and then treated with 25,50μmol·L^-1 G-Rg1 for 24 h.The effect of G-Rg1 on HepG2 cell activity was determined by cell counting kit-8(CCK-8)assay.The level of triglyceride(TG)was detected by micro method.The accumulation of lipid droplets was observed by oil red O staining.Quantitative real-time fluorescence polymerase chain reaction(Real-time PCR)and Western blot were used to detect the alterations of key genes and proteins relating to lipid uptake and metabolism.Result:Compared with the normal group,the intracellular TG level and the absorbance of the oil red O staining in the model group were significantly increased(P<0.01).Compared with the model group,G-Rg1 reduced TG and lipid deposition were significantly reduced(P<0.01).Results of Real-time PCR and Western blot showed that compared with normal group,model group peroxisome proliferators-activated receptors gamma(PPARγ),fatty acid binding protein 1(FABP1),fatty acid transport protein 2/5(FATP2/5)and fatty acid translocase(CD36)expressions increased(P<0.05),whereas peroxisome proliferators-activated receptorsα(PPARα),carnitine palmitoyltransferase 1(CPT1)and peroxisomal acyl-coenzyme A oxidase 1(ACOX1)expressions decreased(P<0.05).Compared with the model group,the expressions of PPARγ,FABP1,FATP2,FATP5 and CD36 in the G-Rg1 group were decreased(P<0.05,P<0.01),while the expressions of PPARα,CPT1 and ACOX1 were increased(P<0.05,P<0.01).Conclusion:G-Rg1 can ameliorate lipid deposition in NAFLD cell model by reducing lipid uptake and increasing lipid oxidation.

关 键 词：人参皂苷RG1 非酒精性脂肪性肝病 HEPG2细胞 脂质代谢 过氧化物酶体增殖物激活受体 

分 类 号：R2-0[医药卫生—中医学] R22