IL-17A对成骨细胞系MC3T3-E1细胞中明胶酶表达的影响及其作用机制  

Effect of IL-17A on the expression of gelatinase in osteoblasts MC3T3-E1 cells and its mechanism

作　　者：杜欣远 宋岩 徐璐 纪雨含 蒋天姿 石塔拉 宓伟[1] DU Xinyuan;SONG Yan;XU Lu;JI Yuhan;JIANG Tianzi;SHI Tala;MI Wei(Nutrition and Food Hygiene Section,Binzhou Medical University,Yantai 264003,Shandong,P.R.China;Department of Stomatology,Municipal Hospital of traditional Chinese Medicine and Shandong Provincial Hospital,Jinan 250021,Shandong)

机构地区：[1]滨州医学院营养与食品卫生学教研室,山东烟台264003 [2]山东省立医院市中医院口腔科,山东济南250021

出　　处：《滨州医学院学报》2020年第3期161-165,共5页Journal of Binzhou Medical University

基　　金：山东省自然科学基金(ZR2017LH059);山东省高校科技计划(J17KA224)。

摘　　要：目的检测IL-17A对MC3T3-E1细胞中明胶酶表达的影响,并探讨其作用机制。方法采用小鼠重组细胞因子IL-17A作用于MC3T3-E1细胞,通过Real-time PCR和ELISA分别检测MMP-2及MMP-9在mRNA水平及蛋白水平上的表达情况;通过免疫荧光和Western Blot检测NF-κB的磷酸化水平的变化,并通过使用NF-κB阻断剂检测其在MMP-9表达中的影响。结果IL-17A在mRNA水平及蛋白水平上均上调MMP-9的表达(P<0.01),然而对MMP-2的表达无明显影响;IL-17A促进转录因子NF-κB的磷酸化水平(P<0.01);阻断NFκB的活性可减弱IL-17A对MMP-9的上调作用(P<0.05);IL-17A对MC3T3-E1细胞中TIMP-1及TIMP-2的表达没有影响。结论IL-17A可以通过激活NF-κB促进MC3T3-E1细胞分泌MMP-9。Objective To investigate the effect of IL-17A on gelatinase expression in MC3T3-E1 cells and to explore its mechanism.Methods MC3T3-E1 cells were stimulated by IL-17A.The expression of MMP-2 and MMP-9 at mRNA and protein levels were detected by Real-time PCR and ELISA respectively.Immunofluorescence and Western Blot were used to detect the IL-17A-induced activity of NF-κB.Chemical inhibitor of NF-κB was used to detect the involvement of NF-κB in IL-17A-induced MMP-9 expression in MC3T3-E1 cells.Results IL-17A upregulated MMP-9 expression at both mRNA and protein levels(P<0.01),but had no effect on MMP-2 expression;IL-17A promoted phosphorylation of NF-κB(P<0.01);inhibition of NF-κB attenuated IL-17A-induced MMP-9 expression(P<0.05).IL-17A did not affect the TIMP-1 and TIMP-2 expression at mRNA level.Conclusion IL-17A can promote the secretion of MMP-9 by MC3T3-E1 cells by activating NF-κB.

关 键 词：白介素-17A 明胶酶 MC3T3-E1 基质金属蛋白酶-9 

分 类 号：R34[医药卫生—基础医学]